alpha Tubulin Monoclonal Antibody
Price:
Size:
500μL
120μL
60μL
30μL
5*60μL
- 宿主: Mouse
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC-p;IF;IP
Verified Samples |
Verified Samples in WB:Hela,Rat brain,Mouse brain Verified Samples in IHC:Mouse liver Verified Samples in IF:Mouse liver |
Dilution | WB 1:500-10000, IHC 1:50-300, IF 1:50-1:200, IP 1:100-1:300 |
Clonality | Monoclonal |
Isotype | IgG |
Immunogen | Recombinant Protein |
Abbre | α-tubulin |
Synonyms | TUBA1A;TUBA3;Tubulin alpha-1A chain;Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain;TUBA1B;Tubulin alpha-1B chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1;Tubulin alpha-ubiquitous chain抗体 |
Swissprot | |
Calculated MW | 50kDa |
Observed MW | 52kDa |
Cellular Localization | Cytoskeleton,cytoplasmic microtubule,microtubule,microtubule cytoskeleton,Cytosol,Endosome,recycling endosome,Extracellular region or secreted,extracellular exosome,Nucleus,Other locations:cytoplasmic ribonucleoprotein granule,membrane raft,myelin sheath |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Purification Method | Protein A purification |
Research Areas | Cancer; Signal Transduction |
Clone No. | Clone:5M5 |
Conjugation | Unconjugated |
Background | There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. |
Western Blot analysis of 1) Hela, 2) Rat brian, 3) Mouse brain using alpha Tubulin Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded mouse liver using alpha Tubulin Monoclonal Antibody at dilution of 1:200
Immunofluorescence analysis of Mouse liver tissue using alpha Tubulin Monoclonal Antibody at dilution of 1:200.
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E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |