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alpha Tubulin Monoclonal Antibody

Price: ¥2835 ¥875 ¥548 ¥295 ¥1975

Size:
500μL 120μL 60μL 30μL 5*60μL
  • 宿主: Mouse
  • 反应性: Human;Mouse;Rat
  • 应用性: WB;IHC-p;IF;IP
Verified Samples
Verified Samples in WB:Hela,Rat brain,Mouse brain
Verified Samples in IHC:Mouse liver
Verified Samples in IF:Mouse liver
Dilution
WB 1:500-10000, IHC 1:50-300, IF 1:50-1:200, IP 1:100-1:300
Clonality
Monoclonal
Isotype
IgG
Immunogen
Recombinant Protein
Abbre
α-tubulin
Synonyms
TUBA1A;TUBA3;Tubulin alpha-1A chain;Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain;TUBA1B;Tubulin alpha-1B chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1;Tubulin alpha-ubiquitous chain抗体
Swissprot
Calculated MW
50kDa
Observed MW
52kDa
Cellular Localization
Cytoskeleton,cytoplasmic microtubule,microtubule,microtubule cytoskeleton,Cytosol,Endosome,recycling endosome,Extracellular region or secreted,extracellular exosome,Nucleus,Other locations:cytoplasmic ribonucleoprotein granule,membrane raft,myelin sheath
Concentration
1 mg/mL
Storage
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method
Protein A purification
Research Areas
Cancer; Signal Transduction
Clone No.
Clone:5M5
Conjugation
Unconjugated
Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
Western Blot analysis of 1) Hela, 2) Rat brian, 3) Mouse brain using alpha Tubulin Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded mouse liver using alpha Tubulin Monoclonal Antibody at dilution of 1:200
Immunofluorescence analysis of Mouse liver tissue using alpha Tubulin Monoclonal Antibody at dilution of 1:200.


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