Anti-Mouse CD162 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)[4RA10]

  • Flow cytometry antibody-Elabscience
  • Flow cytometry antibody-Elabscience
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  • Flow cytometry antibody-Elabscience
  • Flow cytometry antibody-Elabscience
  • Flow cytometry antibody-Elabscience
  • Flow cytometry antibody-Elabscience
    产品货号:E-AB-F1034J

    别称:P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162

    规格:
    • 20 Tests
    • 50 Tests
    • 100 Tests
    • 200 Tests
    数量:
    - +
    价格: ¥1380

    货期: 现货+顺丰周期

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    Antigen Information

    Alternate Names P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162
    Uniprot ID Q62170
    Background The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

    Product Details

    Form Liquid
    Clone No. 4RA10 (See Other Available Formats)
    Host Rat
    Isotype Rat IgG1, κ
    Isotype Control Rat IgG1, κ Isotype Control(PerCP/Cyanine5.5 Conjugated)[HRPN] [Product E-AB-F09822J]
    Reactivity Mouse
    Application FCM (See the Application Description)
    Storage Buffer PBS with 0.05% Proclin300, 1% BSA
    Shipping Biological ice pack at 4 ℃
    Stability & Storage Keep as concentrated solution.
    Store at 2~8℃ and protected from prolonged exposure to light. Do not freeze.
    Centrifuge before opening to ensure complete recovery of vial contents.
    This product is guaranteed up to one year from purchase.

    Fluorophore

    Conjugation: PerCP/Cyanine5.5

    Recommended Use

    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 2 μL or 5 μL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

    Product Data

    • C57BL/6 murine splenocytes are stained with Anti-Mouse CD162 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.

    References

    1. Borges E, Eytner R, Moll T, et al.Blood. 1997; 90(5):1934-1942.
    2. Borges E, Tietz W, Steegmaier M, et al. J Exp Med. 1997; 185(3):573-578.
    3. Frenette PS, Denis CV, Weiss L, et al. . J Exp Med. 2000; 191(8):1413-1422.

    Additional Products

    Catalog No. Product Name Sizes
    E-AB-F1034A Purified Anti-Mouse CD162 Monoclonal Antibody[4RA10] 50 μg, 100 μg
    E-AB-F1034B Anti-Mouse CD162 Monoclonal Antibody(Biotin Conjugated)[4RA10] 50 μg, 100 μg
    E-AB-F1034D Anti-Mouse CD162 Monoclonal Antibody(PE Conjugated)[4RA10] 20 Tests, 50 Tests, 100 Tests, 200 Tests
    E-AB-F1034E Anti-Mouse CD162 Monoclonal Antibody(APC Conjugated)[4RA10] 20 Tests, 50 Tests, 100 Tests, 200 Tests
    E-AB-F1034G Anti-Mouse CD162 Monoclonal Antibody(PE/Cyanine5 Conjugated)[4RA10] 20 Tests, 50 Tests, 100 Tests, 200 Tests
    E-AB-F1034H Anti-Mouse CD162 Monoclonal Antibody(PE/Cyanine7 Conjugated)[4RA10] 20 Tests, 50 Tests, 100 Tests, 200 Tests
    E-AB-F1034I Anti-Mouse CD162 Monoclonal Antibody(PE/Cyanine5.5 Conjugated)[4RA10] 20 Tests, 50 Tests, 100 Tests, 200 Tests
    E-AB-F1034J Anti-Mouse CD162 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)[4RA10] 20 Tests, 50 Tests, 100 Tests, 200 Tests
    E-AB-F1034L Anti-Mouse CD162 Monoclonal Antibody(AF488 Conjugated)[4RA10] 20 Tests, 50 Tests, 100 Tests, 200 Tests
    E-AB-F1034M Anti-Mouse CD162 Monoclonal Antibody(AF647 Conjugated)[4RA10] 20 Tests, 50 Tests, 100 Tests, 200 Tests
    E-AB-F1034UD Anti-Mouse CD162 Monoclonal Antibody(PE Conjugated)[4RA10] 25 μg, 50 μg, 100 μg, 200 μg
    E-AB-F1034UE Anti-Mouse CD162 Monoclonal Antibody(APC Conjugated)[4RA10] 25 μg, 50 μg, 100 μg, 200 μg
    E-AB-F1034UG Anti-Mouse CD162 Monoclonal Antibody(PE/Cyanine5 Conjugated)[4RA10] 25 μg, 50 μg, 100 μg, 200 μg
    E-AB-F1034UH Anti-Mouse CD162 Monoclonal Antibody(PE/Cyanine7 Conjugated)[4RA10] 25 μg, 50 μg, 100 μg, 200 μg
    E-AB-F1034UI Anti-Mouse CD162 Monoclonal Antibody(PE/Cyanine5.5 Conjugated)[4RA10] 25 μg, 50 μg, 100 μg, 200 μg
    E-AB-F1034UJ Anti-Mouse CD162 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)[4RA10] 25 μg, 50 μg, 100 μg, 200 μg
    E-AB-F1034UL Anti-Mouse CD162 Monoclonal Antibody(AF488 Conjugated)[4RA10] 25 μg, 50 μg, 100 μg, 200 μg
    E-AB-F1034UM Anti-Mouse CD162 Monoclonal Antibody(AF647 Conjugated)[4RA10] 25 μg, 50 μg, 100 μg, 200 μg
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