beta actin Monoclonal Antibody

  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
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  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
  • tag control antibody-Elabscience
    产品货号:E-AB-20031

    别称:A26C1A,A26C1B,ACTB,ACTB,Actin beta,Actin cytoplasmic 1,Actin,cytoplasmic 1,N-terminally processed,Actx,b actin,Beta cytoskeletal actin,Beta-actin,BRWS1,E430023M04Rik,MGC128179,PS1TP5 binding protein 1,PS1TP5BP1

    规格:
    • 1mL
    • 500μL
    • 300μL
    • 120μL
    • 60μL
    数量:
    - +
    价格: ¥4800

    宿主: Mouse

    反应性: H,M,R,Mk,Ch,Dg,Hm,Rb,Insect

    应用: WB,IHC-p

    热销!
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    Overview

    Synonyms A26C1A,A26C1B,ACTB,ACTB,Actin beta,Actin cytoplasmic 1,Actin,cytoplasmic 1,N-terminally processed,Actx,b actin,Beta cytoskeletal actin,Beta-actin,BRWS1,E430023M04Rik,MGC128179,PS1TP5 binding protein 1,PS1TP5BP1
    Swissprot P60709
    Source Mouse
    Reactivity Human,Mouse,Rat,Monkey,Chicken,Dog,Hamster,Rabbit,Insect
    Immunogen Synthetic Peptide
    Application WB,IHC-p
    Recommended dilution WB 1:2000-1:5000, IHC 1:50-1:200
    Concentration 1mg/mL
    Clonality Monoclonal

    Properties

    Cellular localization Cytoplasm>cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    Clone No. Clone:2M3
    Isotype IgG
    Purification Protein A purification
    Conjugation Unconjugated
    Storage instructions Store at -20℃. Avoid freeze / thaw cycles.
    Storage buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.4
    Background Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. 

    Images

    • Western Blot analysis of Hela, Rat brain, Mouse brain, Chicken lung, Rabbit testis using beta actin Monoclonal Antibody at dilution of 1:5000.

    • Immunohistochemistry of paraffin-embedded mouse kidney using beta actin Monoclonal Antibody at dilution of 1:200

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