Caspase 9 Activity Assay Kit(Colorimetric Method)
中文名称:Caspase 9活性检测试剂盒(分光光度法)
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- 应用: 细胞凋亡-Caspase
检测原理 | Caspase 9又称ICE-LAP6 或Mch6,是细胞凋亡信号转导过程中比较上游的一个caspase。线粒体释放细胞色素c以后,Caspase 9可以和细胞色素c以及Apaf1形成复合物,同时被激活。激活的caspase 9可以激活细胞凋亡的最关键酶caspase 3,从而促进后续的细胞凋亡信号。Caspase 9的激活可以通过磷酸化进行调控。本caspase 9活性检测试剂盒是基于caspase 9可以催化底物Ac-LEHD-pNA产生黄色的pNA(p-nitroaniline),pNA在405nm附近有强吸收,从而可以通过测定吸光度来检测caspase 9的活性。 |
检测方法 | 比色法 |
样本类型 | 细胞;组织 |
检测时间 | 4-6 hours |
检测仪器 | 分光光度计 |
自备试剂 | PBS(E-BC-R187),Bradford蛋白测定试剂盒(E-BC-K168-S或E-BC-K168-M) |
保存温度 | 该产品可在-20°C避光条件下保存6个月。 |
有效期 | 12个月 |
运输条件 | 冰袋 |
E-CK-A382 | Caspase 2 Activity Assay Kit(Colorimetric Method) | 100Assays , 50Assays , 20Assays |
E-CK-A383 | Caspase 3/7 Activity Assay Kit(Colorimetric Method) | 100Assays , 50Assays , 20Assays |
E-CK-A384 | Caspase 4 Activity Assay Kit(Colorimetric Method) | 100Assays , 50Assays , 20Assays |
Q1:How many cells does Caspase activity kit need? What is the best protein concentration for the assay?
The minimum number of cells is 1 million, and the protein concentration is between 1 and 4 mg/mL.
Q2:Can Caspase series activity kits be used on sf9 cells?
sf9 cells are insect cells, it can be used if caspase is indeed involved in the apoptosis pathway of insect cells.
Q3:Which form of caspase 9 does the kit specifically test? Caspase 9, pro-caspase 9 or cleaved caspase 9?
The Caspase 9 kit measures the enzyme activity of caspase 9, which is activated caspase 9, also known as dimers or a tetramers of cleaved caspase 9.