cGMP(Cyclic GMP) ELISA Kit
中文名称:环磷酸鸟苷(cGMP)酶联免疫吸附测定试剂盒
别称:
cGMP、 3',5'-cyclic GMP、 Guanosine cyclic monophosphate、 Cyclic 3',5'-GMP、 Guanosine 3',5'-cyclic phosphate
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 0.78-50 pmol/mL
- 灵敏度: 0.47 pmol/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用cGMP抗原包被于酶标板上,实验时样品或标准品中的cGMP与包被的cGMP竞争生物素标记的抗cGMP单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,cGMP浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中cGMP的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | 比色法 |
检测范围 | 0.78-50 pmol/mL |
灵敏度 | 0.47 pmol/mL |
样本体积 | 50μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的cGMP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Sildenafil, a Type-5 Phosphodiesterase Inhibitor, Fails to Reverse Myeloid-Derived Suppressor Cell-Mediated T Cell Suppression in Cells Isolated From Tuberculosis Patients
IF: 8.786 -
Effect of bisphenol F on sexual performance and quality of offspring in Male Wistar rats
IF: 7.129 -
Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway
IF: 7.076 -
Glutamine restores testicular glutathione-dependent antioxidant defense and upregulates NO/cGMP signaling in sleep deprivation-induced reproductive dysfunction in rats
IF: 6.53 -
Inhibitory effect of (pro)renin receptor decoy inhibitor PRO20 on endoplasmic reticulum stress during cardiac remodeling
IF: 5.988 -
Inhibition of Phosphodiesterase 5 Promotes the Aromatase-Mediated Estrogen Biosynthesis in Osteoblastic Cells by Activation of cGMP/PKG/SHP2 Pathway
IF: 5.555Journal:Frontiers in Endocrinology -
Inhibition of phosphodiesterase-4 in the spinal dorsal horn ameliorates neuropathic pain via cAMP-cytokine-Cx43 signaling in mice
IF: 5.243Journal:CNS Neuroscience & Therapeutics -
Ziziphus Oxyphylla hydro-methanolic extract ameliorates hypertension in L-NAME induced hypertensive rats through NO/cGMP pathway and suppression of oxidative stress related inflammatory biomarkers.
IF: 4.36Journal:JOURNAL OF ETHNOPHARMACOLOGY -
Protective effect of the combination of essential oil from patchouli and tangerine peel against gastric ulcer in rats
IF: 4.36 -
Bee bread mitigates downregulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet
IF: 4.31Journal:AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISMPMID:34229480