F-TESTO(Free Testosterone) ELISA Kit

中文名称：游离睾酮(F-TESTO)酶联免疫吸附测定试剂盒

货号：E-EL-0165
文献（12）
说明书

说明书

别称：

F-TESTO

Price: ¥2200 ¥1500 ¥9350

Size:

96T 48T 96T*5

反应性： Universal
检测范围： 31.25-2000 pg/mL
灵敏度： 18.75 pg/mL

产品应用	ELISA
检测原理	本试剂盒采用竞争ELISA法。用F-TESTO抗原包被于酶标板上，实验时样品或标准品中的F-TESTO与包被的F-TESTO竞争生物素标记的抗F-TESTO单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，F-TESTO浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中F-TESTO的浓度。
反应类型	Competitive-ELISA
规格	96T / 48T / 96T*5
反应时间	2.5h
反应性	Universal
检测方法	Colormetric
检测范围	31.25-2000 pg/mL
灵敏度	18.75 pg/mL
样本体积	50μL
样本类型	血清、血浆
特异性	可检测样本中的F-TESTO，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃/-20℃
操作步骤
数据处理	ELISA结果计算

Stereological analysis and transcriptome profiling of testicular injury induced by di-(2-ethylhexyl) phthalate in prepubertal rats

IF: 6.291

Journal：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

DOI：10.1016/j.ecoenv.2021.112326

PMID：34015638
Impact of bariatric surgery on androgen profile and ovarian volume in obese polycystic ovary syndrome patients with infertility

IF: 4.219

Journal：SAUDI JOURNAL OF BIOLOGICAL SCIENCES

DOI：10.1016/j.sjbs.2021.05.022

PMID：34466081
Ameliorative Effect of VE, IGF-I, and hCG on the Fluoride-Induced Testosterone Release Suppression in Mice Leydig Cells

IF: 3.738

Journal：BIOLOGICAL TRACE ELEMENT RESEARCH

DOI：10.1007/s12011-017-1023-1
The Role of Mitochondrial Impairment and Oxidative Stress in the Pathogenesis of Lithium-Induced Reproductive Toxicity in Male Mice

IF: 3.412

Journal：Frontiers in Veterinary Science

DOI：10.3389/fvets.2021.603262

PMID：33842567
Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes

IF: 3.227

Journal：REPRODUCTIVE TOXICOLOGY

DOI：10.1016/j.reprotox.2015.07.079
Competitive endogenous RNA (ceRNA) regulation network of lncRNA-miRNA-mRNA during the process of the nickel-induced steroidogenesis disturbance in rat Leydig cells

IF: 3.067

Journal：TOXICOLOGY IN VITRO

DOI：10.1016/j.tiv.2019.104721
Virgin coconut oil extract mitigates testicular‐induced toxicity of alcohol use in antiretroviral therapy

IF: 2.734

Journal：Andrology

DOI：10.1111/andr.12490
Naringenin attenuates highly active antiretroviral therapy‐induced sperm DNA fragmentations and testicular toxicity in Sprague‐Dawley rats

IF: 2.427

Journal：Andrology

DOI：10.1111/andr.12439
Oncostatin M and its receptor in women with polycystic ovary syndrome and association with assisted reproductive technology outcomes

IF: 2.376

Journal：Reproductive Biology

DOI：10.1016/j.repbio.2022.100633

PMID：35278823
Exposure to atrazine during puberty reduces sperm viability, increases weight gain and alters the expression of key metabolic genes in the liver of male mice

IF: 1.723

Journal：REPRODUCTION FERTILITY AND DEVELOPMENT

DOI：10.1071/RD18505

PMID：30636190

Q1:I want to measure the content of testosterone in the supernatant of cell culture. Does the culture medium containing phenol red affect the detection of testosterone?

The medium containing phenol red may have an effect on the detection of testosterone. Phenol red is used in the medium as an indicator of PH: red when neutral, yellow when acidic, and purple when alkaline. Studies have shown that phenol red can mimic the effects of sterol hormones (especially estrogen). To avoid sterol reactions, cultured cells, especially mammalian cells, are cultured without phenol red. Introduce a tip: when the medium is packaged, leave a tube with phenol red and put it in the incubator to balance (you can see the change in PH value, and you can see whether the medium is polluted). Put in the incubator before culture for balanced use (can avoid the use of phenol red during culture).