FITC-11-dUTP
Price:
Size:
250μL
25μL
- 应用： 细胞凋亡-DNA片段化
| 检测原理 | 在细胞凋亡中，染色体DNA的断裂是一个重要的标志事件。凋亡细胞的DNA双链断裂或只要一条链上出现缺口会产生的一系列DNA的3’-OH末端，荧光素标记的dUTP偶联物可在脱氧核糖核苷酸末端转移酶（TdT）的作用下，与断裂DNA的3’-OH末端连接，通过流式细胞仪或荧光显微镜检测到dUTP偶联物发出的荧光。 |
| 检测方法 | 荧光 |
| 样本类型 | 石蜡切片;冰冻切片;细胞爬片;细胞涂片 |
| 检测仪器 | 荧光显微镜 |
| 荧光 | FITC |
| Ex/Em | 490/520 |
| Channel set | FITC |
| Filter set | FITC |
| 保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
Hela cells was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample.(Green)
Paraffin embedded mouse intestinal tissue was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample(Green)
Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.
| E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |
| E-CK-A321 | One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488) | 100Assays , 50Assays , 20Assays |
| E-CK-A322 | One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594) | 100Assays , 50Assays , 20Assays |
| E-CK-A324 | One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 647) | 100Assays , 50Assays , 20Assays |
| E-CK-A325 | One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 555) | 100Assays , 50Assays , 20Assays |
