GCK Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主: Rabbit
- 反应性: Human;Mouse
- 应用性: WB;IHC;IF
Verified Samples |
Verified Samples in WB:Mouse liver Verified Samples in IHC:Mouse liver Verified Samples in IF: HepG2 |
Dilution | WB 1:1000-1:2000 , IHC 1:100-1:200, IF 1:100-1:400 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Recombinant Mouse Glucokinase protein |
Abbre | GCK |
Synonyms | ATP:D-hexose 6-phosphotransferase;FGQTL3;GCK;GK;GLK;Glucokinase;Hexokinase D pancreatic isozyme;Hexokinase type IV;Hexokinase-4;Hexokinase-D;HHF3;HK IV;HK4;HKIV;HXK4;HXKP;LGLK;MODY2抗体 |
Swissprot | |
Calculated MW | 52 kDa |
Observed MW | 52kDa |
Cellular Localization | Nucleus. Cytoplasm. |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% Proclin300 and 50% glycerol, pH7.4. |
Purification Method | Antigen Affinity Purification |
Research Areas | Cancer; Metabolism; Signal Transduction |
Conjugation | Unconjugated |
Background | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). |
Western Blot analysis of Mouse liver using GCK Polyclonal Antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded Mouse liver using GCK Polyclonal Antibody at dilution of 1:100
Immunofluorescence analysis of HepG2 cells using GCK Polyclonal Antibody at dilution of 1:100
E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |