Human ApoE(Apolipoprotein E) ELISA Kit
中文名称：人载脂蛋白E(ApoE)酶联免疫吸附测定试剂盒
Apo-E、MGC1571、Apolipoprotein E3
Price:
- 反应性： Human
- 检测范围： 23.44-1500 ng/mL
- 灵敏度： 14.06 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人ApoE抗体包被于酶标板上，实验时样品（或标准品）中的人ApoE会与包被抗体结合。后依次加入生物素化的抗人ApoE抗体和辣根过氧化物酶标记的亲和素，抗人ApoE抗体与结合在包被抗体上的人ApoE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ApoE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ApoE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96T
/ 48T
/ 96T*5
|
| 反应时间 | 3.5h |
| 反应性 | Human |
| 检测方法 | Colormetric |
| 检测范围 | 23.44-1500 ng/mL |
| 灵敏度 | 14.06 ng/mL |
| 样本体积 | 100μL |
| 样本类型 | 血清，血浆，细胞上清，细胞提取液体，组织匀浆等其他生物体液 |
| 特异性 | 可检测样本中的人ApoE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃/-20℃ |
| 数据处理 |
-
Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory
IF: 17.173 -
Safety and Effects of Lactobacillus paracasei TISTR 2593 Supplementation on Improving Cholesterol Metabolism and Atherosclerosis-Related Parameters in Subjects with Hypercholesterolemia: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial
IF: 6.706Journal：Nutrients -
Bioinformatic Analysis of the Proteome in Exosomes Derived From Plasma: Exosomes Involved in Cholesterol Metabolism Process of Patients With Spinal Cord Injury in the Acute Phase
IF: 4.081Journal：Frontiers in Neuroinformatics -
APOE genotypes, lipid profiles and associated clinical markers in a Finnish population with cardiovascular disease risk factors
IF: 2.5Journal：Lifestyle Genomics -
Evaluation of α-synuclein and apolipoprotein E as potential biomarkers in cerebrospinal fluid to monitor pharmacotherapeutic efficacy in dopamine dictated disease states of Parkinson’s disease and schizophrenia
IF: 2.228Journal：Neuropsychiatric Disease and Treatment
Q1:Human ApoE(Apolipoprotein E) ELISA Kit This can be directly seen as ApoE 3/4 of the gene?
According to the ApoE gene model, the synthesis of ApoE is controlled by three alleles located at a single gene locus, namely E2, E3 and E4. Each allele corresponds to a major isomer to produce three homozygotes (E2/2, E3/3, E4/4) and three heterozygotes (E2/3, E2/4, E3/4), a total of six common phenotypes. ApoE 3/4 is at the gene level, and ELISA kit detects the APOE content at the protein level, which cannot reflect the expression of APOE3/4 at the gene level.
