Human BMP-4(Bone Morphogenetic Protein 4) ELISA Kit
中文名称:人骨成型蛋白4(BMP-4)酶联免疫吸附测定试剂盒
别称:
BMP2B、BMP2B1、MCOPS6、ZYME、DVR4、OFC11、ZYME
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人BMP-4抗体包被于酶标板上,实验时样品(或标准品)中的人BMP-4会与包被抗体结合。后依次加入生物素化的抗人BMP-4抗体和辣根过氧化物酶标记的亲和素,抗人BMP-4抗体与结合在包被抗体上的人BMP-4结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,BMP-4浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中BMP-4的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人BMP-4,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Early Osteogenic Marker Expression in hMSCs Cultured onto Acid Etching-Derived Micro- and Nanotopography 3D-Printed Titanium Surfaces
IF: 6.208 -
The Attenuating Effect of the Intraovarian Bone Morphogenetic Protein 4 on Age-Related Endoplasmic Reticulum Stress in Chicken Follicular Cells
IF: 5.076Journal:Oxidative Medicine and Cellular Longevity -
Comparative effects of virtual reality training and sensory motor training on bone morphogenic proteins and inflammatory biomarkers in post-traumatic osteoarthritis
IF: 3.998 -
Correlation of the BMP-4 and BMP-7 Proteins Concentration and Expression in Response to a Specific and Non-Specific Trigger in Asthma
Journal:Advances in Respiratory Medicine -
Prognostic Value of Bone Formation and Resorption Proteins in Heterotopic Ossification in Critically-Ill Patients. a Single-Centre Study.
Journal:Journal of Critical Care Medicine