Human ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit
中文名称:人内皮细胞特异分子1(ESM1)酶联免疫吸附测定试剂盒
别称:
endocan、Endothelial cell-specific molecule 1
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人ESM1抗体包被于酶标板上,实验时样品(或标准品)中的人ESM1会与包被抗体结合。后依次加入生物素化的抗人ESM1抗体和辣根过氧化物酶标记的亲和素,抗人ESM1抗体与结合在包被抗体上的人ESM1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,ESM1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中ESM1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人ESM1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Increased Carotid Intima–Media Thickness and Endothelial Cell-Specific Molecule-1 (Endocan) Levels in Obese Children:
IF: 3.619Journal:ANGIOLOGY -
Endocan: A Novel Predictor of Endothelial Dysfunction in Silent Brain Infarction:
IF: 3.619Journal:ANGIOLOGY -
Effect of Non‐Surgical Periodontal Treatment on Gingival Crevicular Fluid and Serum Endocan, Vascular Endothelial Growth Factor‐A, and Tumor Necrosis Factor‐Alpha Levels
IF: 3.03Journal:JOURNAL OF PERIODONTOLOGY -
Serum endocan levels in patients with stable COPD
IF: 2.917Journal:International Journal of Chronic Obstructive Pulmonary Disease -
Maternal Serum Endocan Concentration in Pregnancies Complicated by Intrauterine Growth Restriction:
IF: 2.548Journal:Reproductive Sciences -
The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer
IF: 2.447 -
The investigation of levels of endothelial cell-specific molecule, progranuline, clusterin, and human epididymis protein 4 in the differential diagnosis of malignant pleural effusions
IF: 1.817Journal:MEDICINE -
The utility of maternal serum endocan level to predict preterm delivery within seven days in patients with threatened preterm labor
IF: 1.569Journal:Journal of Maternal-Fetal & Neonatal Medicine -
Serum endocan concentration and its correlation with severity of hypertensive disorders in pregnancy
IF: 1.493Journal:Journal of Maternal-Fetal & Neonatal MedicinePMID:30501553 -
Maternal serum endocan concentrations are elevated in patients with preterm premature rupture of membranes
IF: 1.361