Human GAL3(Galectin 3) ELISA Kit
中文名称:人半乳凝集素3(GAL3)酶联免疫吸附测定试剂盒
别称:
LGALS3、CBP35、GALBP、GALIG、L31、LGALS2、MAC2、Lectin、Galactoside-Binding Soluble 3
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人GAL3抗体包被于酶标板上,实验时样品(或标准品)中的人GAL3会与包被抗体结合。后依次加入生物素化的抗人GAL3抗体和辣根过氧化物酶标记的亲和素,抗人GAL3抗体与结合在包被抗体上的人GAL3结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,GAL3浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中GAL3的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人GAL3,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Evaluation of galectin-3, peptidylarginine deiminase-4 and tumor necrosis factor-α levels in gingival crevicular fluid for periodontal health, gingivitis and stage III grade C periodontitis: A pilot study
IF: 6.993 -
FK506-Binding Protein like (FKBPL) Has an Important Role in Heart Failure with Preserved Ejection Fraction Pathogenesis with Potential Diagnostic Utility
IF: 6.064Journal:Biomolecules -
Imaging, biomarker and invasive assessment of diffuse left ventricular myocardial fibrosis in atrial fibrillation
IF: 5.361 -
Clinical Value of Circulating Microribonucleic Acids miR-1 and miR-21 in Evaluating the Diagnosis of Acute Heart Failure in Asymptomatic Type 2 Diabetic Patients.
IF: 4.694Journal:Biomolecules -
Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event
IF: 4.141Journal:Genes -
Diagnostic Performance of Serum Biomarkers Fibroblast Growth Factor 21, Galectin-3 and Copeptin for Heart Failure with Preserved Ejection Fraction in a Sample of Patients with Type 2 Diabetes Mellitus
IF: 3.706 -
How Diabetes and Other Comorbidities of Elderly Patients and Their Treatment Influence Levels of Glycation Products
IF: 3.39Journal:International Journal of Environmental Research and Public HealthPMID:35742776 -
Elevated β1-Adrenergic Receptor Autoantibody Levels Increase Atrial Fibrillation Susceptibility by Promoting Atrial Fibrosis
IF: 3.367Journal:Frontiers in Physiology -
Galectin‑3 facilitates the proliferation and migration of nasopharyngeal carcinoma cells via activation of the ERK1/2 and Akt signaling pathways, and is positively correlated with the inflammatory state of nasopharyngeal carcinoma
IF: 2.952 -
Changes in Left Heart Geometry, Function, and Blood Serum Biomarkers in Patients with Obstructive Sleep Apnea after Treatment with Continuous Positive Airway Pressure
IF: 2.948