Human GHRL(Ghrelin) ELISA Kit
中文名称:人饥饿素(GHRL)酶联免疫吸附测定试剂盒
别称:
GHRL、MTLRP、Ghrelin
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用人GHRL抗原包被于酶标板上,实验时样品或标准品中的人GHRL与包被的人GHRL竞争生物素标记的抗人GHRL单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,人GHRL浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中人GHRL的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 50μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人GHRL,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
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Effectiveness of a polyphenolic extract (Lippia citriodora and Hibiscus sabdariffa) on appetite regulation in overweight and obese grade I population: an 8-week randomized, double-blind, cross-over, placebo-controlled trial
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Associations between serum ghrelin and knee symptoms, joint structures and cartilage or bone biomarkers in patients with knee osteoarthritis
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Acute and short-term effects of Lactobacillus paracasei subsp. paracasei 431 and inulin intake on appetite control and dietary intake: A two-phases randomized, double blind, placebo-controlled study
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The effect of umbilical cord blood spexin, free 25(OH) vitamin D3 and adipocytokine levels on intrauterine growth and anthropometric measurements in newborns
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A Case Control Study on Serum Levels of Potential Biomarkers in Male Breast Cancer Patients
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Changes in ghrelin, substance P and vasoactive intestinal peptide levels in the gastroduodenal mucosa of patients with morbid obesity
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High Ghrelin Level Predicts the Curve Progression of Adolescent Idiopathic Scoliosis Girls
IF: 2.583Journal:Biomed Research International -
The association of serum nesfatin-1 and ghrelin levels with metabolic syndrome in patients with schizophrenia
IF: 2.528Journal:PSYCHIATRY RESEARCH -
Effect of Sequence of Fruit Intake in a Meal on Satiety
IF: 2.468Journal:International Journal of Environmental Research and Public HealthPMID:31766283