Human IFN-β(Interferon Beta) ELISA Kit
中文名称:人β干扰素(IFN-β)酶联免疫吸附测定试剂盒
别称:
IFNB1、IFB、IFF、IFNB
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-β抗体包被于酶标板上,实验时样品(或标准品)中的人IFN-β会与包被抗体结合。后依次加入生物素化的抗人IFN-β抗体和辣根过氧化物酶标记的亲和素,抗人IFN-β抗体与结合在包被抗体上的人IFN-β结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IFN-β浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFN-β的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人IFN-β,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Differential immune responses in pregnant patients recovered from COVID-19
IF: 18.187 -
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
IF: 11.556 -
CD47-targeted immunotherapy unleashes antitumour immunity in Epstein-Barr virus-associated gastric cancer
IF: 10.19Journal:CLINICAL IMMUNOLOGY -
Necroptotic virotherapy of oncolytic alphavirus M1 cooperated with Doxorubicin displays promising therapeutic efficacy in TNBC
IF: 9.867 -
Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion
IF: 8.469 -
The EF-Hand Protein CALML6 Suppresses Antiviral Innate Immunity by Impairing IRF3 Dimerization
IF: 7.815 -
β-adrenergic receptor inhibition enhances oncolytic herpes virus propagation through STAT3 activation in gastric cancer
IF: 7.133 -
JMJD6 negatively regulates cytosolic RNA induced antiviral signaling by recruiting RNF5 to promote activated IRF3 K48 ubiquitination
IF: 6.823 -
The C-Terminal Effector Domain of Non-Structural Protein 1 of Influenza A Virus Blocks IFN-β Production by Targeting TNF Receptor-Associated Factor 3
IF: 6.429Journal:Frontiers in Immunology -
Influenza A Virus PA Antagonizes Interferon-β by Interacting with Interferon Regulatory Factor 3
IF: 6.429Journal:Frontiers in Immunology