Human IFN-γ(Interferon Gamma) ELISA Kit
中文名称:人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
别称:
IFNG、IFG、IFI、Type II Interferon
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上,实验时样品(或标准品)中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素,抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IFN-γ浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人IFN-γ,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils
IF: 5.546 -
A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of gut microbiota and relief from chronic constipation
IF: 5.396Journal:Food & Function -
Immunosuppression Induced by Glutamine Deprivation Occurs via Activating PD-L1 Transcription in Bladder Cancer
IF: 5.246 -
Glucose metabolism involved in PD-L1-mediated immune escape in the malignant kidney tumour microenvironment
IF: 5.241 -
Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis
IF: 4.996Journal:Scientific Reports -
Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding
IF: 4.932 -
Platelet lysate supports the in vitro expansion of human periodontal ligament stem cells for cytotherapeutic use
IF: 4.71Journal:Journal of Tissue Engineering and Regenerative Medicine -
Decreased expression of programmed death 1 on peripheral blood lymphocytes disrupts immune homeostasis in peripartum cardiomyopathy
IF: 4.638Journal:INTERNATIONAL JOURNAL OF CARDIOLOGY -
Glutamine Deprivation Induces PD-L1 Expression via Activation of EGFR/ERK/c-Jun Signaling in Renal Cancer
IF: 4.63 -
Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis
IF: 4.575