Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit
中文名称:人胰岛素样生长因子1(IGF-1)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IGF-1抗体包被于酶标板上,实验时样品(或标准品)中的人IGF-1会与包被抗体结合。后依次加入生物素化的抗人IGF-1抗体和辣根过氧化物酶标记的亲和素,抗人IGF-1抗体与结合在包被抗体上的人IGF-1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IGF-1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IGF-1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人IGF-1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
LncCCLM inhibits lymphatic metastasis of cervical cancer by promoting STAU1-mediated IGF-1 mRNA degradation
IF: 8.679 -
Insulin-like growth factor-1 attenuates oxidative stress-induced hepatocyte premature senescence in liver fibrogenesis via regulating nuclear p53–progerin interaction
IF: 5.959Journal:Cell Death & Disease -
Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway
IF: 5.443 -
CTCF variant begets to short stature by down-regulation of IGF-1
IF: 4.869Journal:JOURNAL OF MOLECULAR ENDOCRINOLOGY -
MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition
IF: 4.831 -
Prognostic Significance of Serum Insulin-Like Growth Factor-1 in Hepatocellular Cancer Patients: A Validation Study
IF: 4.655Journal:Journal of Hepatocellular Carcinoma -
Obesity-related proteins score as a potential marker of breast cancer risk
IF: 4.38 -
Comparison of Serum VEGF, IGF-1, and HIF-1α Levels in Children with Autism Spectrum Disorder and Healthy Controls
IF: 4.291 -
A Novel 7-Days Prolonged Dietary Deprivation Regimen Improves ALT and UA After 3–6 Months Refeeding, Indicating Therapeutic Potential
IF: 3.365Journal:Frontiers in Nutrition -
Ramadan diurnal intermittent fasting modulates SOD2, TFAM, Nrf2, and sirtuins (SIRT1, SIRT3) gene expressions in subjects with overweight and obesity
IF: 3.239Journal:DIABETES RESEARCH AND CLINICAL PRACTICE
E-OSEL-H0012 | QuicKey Pro Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit | 96T , 48T , 96T*5 |