Human IL-33(Interleukin 33) ELISA Kit
中文名称:人白介素33(IL-33)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-33抗体包被于酶标板上,实验时样品(或标准品)中的人IL-33会与包被抗体结合。后依次加入生物素化的抗人IL-33抗体和辣根过氧化物酶标记的亲和素,抗人IL-33抗体与结合在包被抗体上的人IL-33结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-33浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-33的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人IL-33,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
IF: 11.556 -
Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis
IF: 4.575 -
Systemic and local cytokine profile and risk factors for persistent allergic airway inflammation in patients sensitised to house dust mite allergens
IF: 3.317 -
A role for serum cytokines and cell adhesion molecules in the non‑invasive diagnosis of colorectal cancer
IF: 3.111Journal:Oncology Letters -
The function of IL-33/ST2 signaling axis in treg cells activating fibrosis in IgG4-related disease
IF: 2.85 -
Anti-IgE Significantly Changes Circulating Interleukin-25, Vitamin-D and Interleukin-33 Levels in Patients with Allergic Asthma
IF: 2.412 -
A case of asthma with Behcet’s disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions
IF: 2.352Journal:IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY -
Wheat‐dependent exercise‐induced anaphylaxis in Chinese people: a clinical research on 33 cases with antigenic analysis of wheat proteins
IF: 1.771Journal:CLINICAL AND EXPERIMENTAL DERMATOLOGY -
Increased TSLP, IL-33, IL-25, IL-19, IL 21 and amphiregulin (AREG) levels in chronic rhinosinusitis with nasal polyp
IF: 1.75 -
Could interleukin-33 and its suppressor of tumorigenicity 2 (ST2) receptor have a role in cervical human papillomavirus (HPV) infections?
IF: 1.406