Human INS(Insulin) ELISA Kit
中文名称:人胰岛素(INS)酶联免疫吸附测定试剂盒
别称:
IDDM2、ILPR、IRDN、MODY10
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.78-50 μIU/mL
- 灵敏度: 0.47 μIU/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人INS抗体包被于酶标板上,实验时样品(或标准品)中的人INS会与包被抗体结合。后依次加入生物素化的抗人INS抗体和辣根过氧化物酶标记的亲和素,抗人INS抗体与结合在包被抗体上的人INS结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,INS浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中INS的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法 |
检测范围 | 0.78-50 μIU/mL |
灵敏度 | 0.47 μIU/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人INS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
The effect of hydrothermal processing on the performance of broiler chicks fed corn or sorghum-based diets
IF: 3.247Journal:ANIMAL FEED SCIENCE AND TECHNOLOGY -
Ramadan diurnal intermittent fasting modulates SOD2, TFAM, Nrf2, and sirtuins (SIRT1, SIRT3) gene expressions in subjects with overweight and obesity
IF: 3.239Journal:DIABETES RESEARCH AND CLINICAL PRACTICE -
Metabolomics and correlation network analyses of core biomarkers in type 2 diabetes
IF: 3.063 -
Ramadan Intermittent Fasting Is Associated with Changes in Circulating Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) in Metabolically Healthy Obese Subjects
IF: 2.948Journal:Medicina-Lithuania -
Insulin Resistance is Associated with Gut Permeability Without the Direct Influence of Obesity in Young Adults
IF: 2.842Journal:Diabetes Metabolic Syndrome and Obesity-Targets and TherapyPMID:32922055 -
Serum profile of cytokines and their genetic variants in metabolic syndrome and healthy subjects: a comparative study
IF: 2.535Journal:BIOSCIENCE REPORTS -
Fermented soybean enhances post-meal response in appetite-regulating hormones among Indonesian girls with obesity
IF: 2.288 -
Effect of Lycium barbarum polysaccharide supplementation in non-alcoholic fatty liver disease patients: study protocol for a randomized controlled trial
IF: 2.279 -
Assessment of Serum Chemerin Levels in Acanthosis Nigricans: A Case Control Study
IF: 2.189Journal:Journal of Cosmetic Dermatology -
Effect of Preoperative Oral Carbohydrate Administration on Patients Undergoing Cesarean Section with Epidural Anesthesia: A Pilot Study
IF: 0.805