Human IP-10/CXCL10(Interferon Gamma Induced Protein 10kDa) ELISA Kit
中文名称:人10kDa干扰素γ诱导蛋白(IP-10/CXCL10)酶联免疫吸附测定试剂盒
别称:
C7、IFI10、INP10、SCYB10、Crg-2、GIP-10、Mob-1
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IP-10/CXCL10抗体包被于酶标板上,实验时样品(或标准品)中的人IP-10/CXCL10会与包被抗体结合。后依次加入生物素化的抗人IP-10/CXCL10抗体和辣根过氧化物酶标记的亲和素,抗人IP-10/CXCL10抗体与结合在包被抗体上的人IP-10/CXCL10结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IP-10/CXCL10浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IP-10/CXCL10的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人IP-10/CXCL10,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
IF: 11.556 -
Pathogenic Roles of CXCL10 in Experimental Autoimmune Prostatitis by Modulating Macrophage Chemotaxis and Cytokine Secretion
IF: 7.561 -
Inflammation-Driven Secretion Potential Is Upregulated in Osteoarthritic Fibroblast-Like Synoviocytes
IF: 6.208 -
Activation of the STING-IRF3 pathway involved in psoriasis with diabetes mellitus
IF: 5.31Journal:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE -
Leptin correlates with monocytes activation and severe condition in COVID-19 patients
IF: 4.962 -
Diosmectite inhibits the interaction between SARS-CoV-2 and human enterocytes by trapping viral particles, thereby preventing NF-kappaB activation and CXCL10 secretion
IF: 4.38 -
Exploring alternative cytokines as potential biomarkers for latent tuberculosis infection in pregnant women
IF: 3.752