Human MHCG/HLA-G(Major Histocompatibility Complex Class Ⅰ G) ELISA Kit
中文名称:人Ⅰ类主要组织相容性复合体G(MHCG/HLA-G)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.63-40 ng/mL
- 灵敏度: 0.38 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MHCG/HLA-G抗体包被于酶标板上,实验时样品(或标准品)中的人MHCG/HLA-G会与包被抗体结合。后依次加入生物素化的抗人MHCG/HLA-G抗体和辣根过氧化物酶标记的亲和素,抗人MHCG/HLA-G抗体与结合在包被抗体上的人MHCG/HLA-G结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,MHCG/HLA-G浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MHCG/HLA-G的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.63-40 ng/mL |
灵敏度 | 0.38 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人MHCG/HLA-G,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Downregulated cytotoxic CD8+ T-cell identifies with the NKG2A-soluble HLA-E axis as a predictive biomarker and potential therapeutic target in keloids
IF: 11.53 -
The Potential of Soluble Human Leukocyte Antigen Molecules for Early Cancer Detection and Therapeutic Vaccine Design
IF: 4.086 -
Understanding HLA-G driven journey from HPV infection to cancer cervix: Adding missing pieces to the jigsaw puzzle
IF: 4.018 -
Circulating fetal and total cell-free DNA, and sHLA-G in black South African women with gestational hypertension and pre-eclampsia
IF: 1.099Journal:HYPERTENSION IN PREGNANCY -
Susceptibility role of soluble HLA-G and HLA-G 14-bp insertion/deletion polymorphism in inflammatory bowel disease
Journal:Egyptian Journal of Medical Human Genetics -
Evaluation of sHLA-G levels in serum of patients with prostate cancer identify as a potential of tumor marker
Journal:Anatomy & Cell Biology