Human MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称:人基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MMP-9抗体包被于酶标板上,实验时样品(或标准品)中的人MMP-9会与包被抗体结合。后依次加入生物素化的抗人MMP-9抗体和辣根过氧化物酶标记的亲和素,抗人MMP-9抗体与结合在包被抗体上的人MMP-9结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,MMP-9浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MMP-9的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人MMP-9,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
MMP2/MMP9-mediated CD100 shedding is crucial for inducing intrahepatic anti-HBV CD8 T cell responses and HBV clearance
IF: 18.946 -
Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers
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Quantitative phosphoproteomic analysis identifies the critical role of JNK1 in neuroinflammation induced by Japanese encephalitis virus
IF: 7.359Journal:Science Signaling -
The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases
IF: 6.6Journal:Cells -
Dual-Sensitive Fluorescent Nanoprobes for Detection of Matrix Metalloproteinases and Low pH in 3D Tumor Microenvironment
IF: 6.331Journal:Journal of Materials Chemistry B -
Mechanisms of Antitumor Invasion and Metastasis of the Marine Fungal Derivative Epi-Aszonalenin A in HT1080 Cells
IF: 6.085Journal:Marine Drugs -
Hypermethylation of the Keap1 gene inactivates its function, promotes Nrf2 nuclear accumulation, and is involved in arsenite-induced human keratinocyte transformation
IF: 5.736Journal:FREE RADICAL BIOLOGY AND MEDICINE -
Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo
IF: 5.65Journal:INTERNATIONAL JOURNAL OF ONCOLOGY -
A novel glyceroglycolipid from brown algae Ishige okamurae improve photoaging and counteract inflammation in UVB-induced HaCaT cells
IF: 5.194 -
Infection of Mycobacterium tuberculosis Promotes Both M1/M2 Polarization and MMP Production in Cigarette Smoke-Exposed Macrophages
IF: 5.085