Human MT(Melatonin) ELISA Kit
中文名称:人褪黑素(MT)酶联免疫吸附测定试剂盒
别称:
N-Acetyl-5-Methoxytryptamine
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用人MT抗原包被于酶标板上,实验时样品或标准品中的人MT与包被的人MT竞争生物素标记的抗人MT单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,人MT浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中人MT的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 50μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人MT,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Measuring melatonin by immunoassay
IF: 14.528Journal:JOURNAL OF PINEAL RESEARCH -
Melatonin enhances osteoblastogenesis of senescent bone marrow stromal cells through NSD2-mediated chromatin remodelling
IF: 11.492Journal:Clinical and Translational MedicineDOI:10.1002/ctm2.746 -
Increased Expression of Colonic Mucosal Melatonin in Patients with Irritable Bowel Syndrome Correlated with Gut Dysbiosis
IF: 7.691 -
Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway
IF: 6.6 -
Sleep duration of lactating mothers and its relationship with feeding pattern, milk macronutrients and related serum factors: A combined longitudinal cohort and cross-sectional study
IF: 6.59 -
Peripheral Clock System Abnormalities in Patients With Parkinson’s Disease
IF: 5.75Journal:Frontiers in Aging Neuroscience -
Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway
IF: 5.719 -
Melatonin exerts immunoregulatory effects by balancing peripheral effector and regulatory T helper cells in myasthenia gravis
IF: 4.831 -
Yoga improves mitochondrial health and reduces severity of autoimmune inflammatory arthritis: A randomized controlled trial
IF: 4.16 -
Melatonin regulates proliferation, apoptosis and invasion of trophoblasts in preeclampsia by inhibiting endoplasmic reticulum stress
IF: 3.886