Human MUC2(Mucin 2) ELISA Kit
中文名称:人粘蛋白2(MUC2)酶联免疫吸附测定试剂盒
别称:
MLP、MUC-2、SMUC
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.31-20 ng/mL
- 灵敏度: 0.19 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MUC2抗体包被于酶标板上,实验时样品(或标准品)中的人MUC2会与包被抗体结合。后依次加入生物素化的抗人MUC2抗体和辣根过氧化物酶标记的亲和素,抗人MUC2抗体与结合在包被抗体上的人MUC2结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,MUC2浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MUC2的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 0.31-20 ng/mL |
灵敏度 | 0.19 ng/mL |
样本体积 | 100μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人MUC2,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Hybrid Lipid/Polymer Nanoparticles to Tackle the Cystic Fibrosis Mucus Barrier in siRNA Delivery to the Lungs: Does PEGylation Make the Difference?
IF: 9.229 -
Specific activation of hypoxia-inducible factor-2α by propionate metabolism via a β-oxidation-like pathway stimulates MUC2 production in intestinal goblet cells
IF: 7.419 -
Melatonin restores Muc2 depletion induced by V. vulnificus VvpM via melatonin receptor 2 coupling with Gαq
IF: 5.762Journal:JOURNAL OF BIOMEDICAL SCIENCE -
Xenogeneic-Free Human Intestinal Organoids for Assessing Intestinal Nutrient Absorption
IF: 5.719 -
Antimicrobial Peptides Human Beta-Defensin-2 and -3 Protect the Gut During Candida albicans Infections Enhancing the Intestinal Barrier Integrity: In Vitro Study
IF: 5.293Journal:Frontiers in Cellular and Infection Microbiology -
Shaoyao decoction restores the mucus layer in mice with DSS-induced colitis by regulating Notch signaling pathway
IF: 5.195Journal:JOURNAL OF ETHNOPHARMACOLOGY -
Asthma with bronchial hypersecretion: expression of mucins and toll-like receptors in sputum and blood
IF: 4.258Journal:Journal of Asthma and Allergy -
Exogenous NAD+ Stimulates MUC2 Expression in LS 174T Goblet Cells via the PLC-Delta/PTGES/PKC-Delta/ERK/CREB Signaling Pathway
IF: 4.082Journal:Biomolecules -
Dairy Propionibacterium freudenreichii ameliorates acute colitis by stimulating MUC2 expression in intestinal goblet cell in a DSS-induced colitis rat model
IF: 3.998Journal:Scientific Reports