Human MUC5AC(Mucin 5 Subtype AC) ELISA Kit
中文名称:人粘蛋白5AC(MUC5AC)酶联免疫吸附测定试剂盒
别称:
mucin-5AC、MUC5、Gastric mucin|mucin 5、Subtypes A and C、Tracheobronchial/gastric|mucin 5AC|tracheobronchial mucin
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.31-20 ng/mL
- 灵敏度: 0.19 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MUC5AC抗体包被于酶标板上,实验时样品(或标准品)中的人MUC5AC会与包被抗体结合。后依次加入生物素化的抗人MUC5AC抗体和辣根过氧化物酶标记的亲和素,抗人MUC5AC抗体与结合在包被抗体上的人MUC5AC结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,MUC5AC浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MUC5AC的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法 |
检测范围 | 0.31-20 ng/mL |
灵敏度 | 0.19 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人MUC5AC,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Hybrid Lipid/Polymer Nanoparticles to Tackle the Cystic Fibrosis Mucus Barrier in siRNA Delivery to the Lungs: Does PEGylation Make the Difference?
IF: 9.229 -
NICD3 regulates the expression of MUC5AC and MUC2 by recruiting SMARCA4 and is involved in the differentiation of mucinous colorectal adenocarcinoma
IF: 7.449 -
Altered Ocular Surface Health Status and Tear Film Immune Profile Due to Prolonged Daily Mask Wear in Health Care Workers
IF: 6.081 -
Keeping Candida commensal: how lactobacilli antagonize pathogenicity of Candida albicans in an in vitro gut model
IF: 4.028 -
MicroRNA-218 acts by repressing TNFR1-mediated activation of NF-κB, which is involved in MUC5AC hyper-production and inflammation in smoking-induced bronchiolitis of COPD
IF: 3.858Journal:TOXICOLOGY LETTERS -
In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extracts
IF: 2.406Journal:PULMONARY PHARMACOLOGY & THERAPEUTICS