Human NFKB-p65(Nuclear Factor Kappa B p65) ELISA Kit
中文名称:人核因子κB亚基p65(NFKB-p65)酶联免疫吸附测定试剂盒
别称:
NFKB3、P65、RelA
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人NFKB-p65抗体包被于酶标板上,实验时样品(或标准品)中的人NFKB-p65会与包被抗体结合。后依次加入生物素化的抗人NFKB-p65抗体和辣根过氧化物酶标记的亲和素,抗人NFKB-p65抗体与结合在包被抗体上的人NFKB-p65结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,NFKB-p65浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中NFKB-p65的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人NFKB-p65,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Traditional Chinese Medicine, Qingfei Paidu Decoction and Xuanfei Baidu Decoction, Inhibited Cytokine Production via NF-κB Signaling Pathway in Macrophages: Implications for Coronavirus Disease 2019 (COVID-19) Therapy
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Low-dose 5-aza-2′-deoxycytidine protects against early renal injury by increasing klotho expression
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Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kβsignaling
IF: 4.018 -
Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production
IF: 3.84 -
Early Diagnosis of Coronary Artery Disease by Inflammatory Biomarkers of Atherosclerosis in Patients with Angina
IF: 3.657 -
Triclosan affects the expression of nitric oxide synthases (NOSs), peroxisome proliferator-activated receptor gamma (PPARγ), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in mouse neocortical neurons in vitro
IF: 3.5 -
Persicaria minor (Huds.) Opiz Prevents In Vitro Atherogenesis by Attenuating Tumor Necrosis Factor-α-Induced Monocyte Adhesion to Human Umbilical Vein Endothelial Cells
IF: 3.251Journal:Life-Basel -
Embelin induces apoptosis of human gastric carcinoma through inhibition of p38 MAPK and NF-κB signaling pathways
IF: 1.559Journal:Molecular Medicine Reports