Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
中文名称:人中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫吸附测定试剂盒
别称:
LCN2、Lipocalin 2、Oncogene 24p3、MSFI
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人NGAL抗体包被于酶标板上,实验时样品(或标准品)中的人NGAL会与包被抗体结合。后依次加入生物素化的抗人NGAL抗体和辣根过氧化物酶标记的亲和素,抗人NGAL抗体与结合在包被抗体上的人NGAL结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,NGAL浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中NGAL的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法 |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人NGAL,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Identification of key candidate biomarkers for severe influenza infection by integrated bioinformatical analysis and initial clinical validation
IF: 5.31 -
Biomarkers of Uremic Cardiotoxicity
IF: 4.546 -
Gingival crevicular fluid lipocalin-2 and semaphorin3A in stage III periodontitis: Non-surgical periodontal treatment effects
IF: 4.419 -
Plasma lipocalin-2/NGAL is stable over 12 weeks and is not modulated by exercise or dieting
IF: 4.38Journal:Scientific Reports -
Renal Stem Cells, Renal Resistive Index, and Neutrophil Gelatinase Associated Lipocalin Changes After Revascularization in Patients With Renovascular Hypertension and Ischemic Nephropathy
IF: 3.31Journal:CURRENT PHARMACEUTICAL DESIGN -
Effects of pneumoperitoneum on kidney injury biomarkers: A randomized clinical trial
IF: 3.24 -
Urinary NGAL, IGFBP-7, and TIMP-2: novel biomarkers to predict contrast medium-induced acute kidney injury in children
IF: 3.222 -
Serum neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for predicting high dose methotrexate associated acute kidney injury in children with acute lymphoblastic leukemia
IF: 3.008 -
Association of plasma neutrophil gelatinase-associated lipocalin with parameters of CKD–MBD in maintenance hemodialysis patients
IF: 2.626Journal:JOURNAL OF BONE AND MINERAL METABOLISM -
Assessment of acute aerobic exercise in the morning versus evening on asprosin, spexin, lipocalin-2, and insulin level in overweight/obese versus normal weight adult men
IF: 2.486
E-TSEL-H0003 | QuicKey Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit | 96T , 48T , 96T*5 |