Human SDF-1/CXCL12(Stromal Cell Derived Factor 1) ELISA Kit
中文名称:人基质细胞衍生因子1(SDF-1/CXCL12)酶联免疫吸附测定试剂盒
别称:
SDF1、CXCL12、IRH、PBSF、SCYB12、TLSF、TPAR1
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人SDF-1/CXCL12抗体包被于酶标板上,实验时样品(或标准品)中的人SDF-1/CXCL12会与包被抗体结合。后依次加入生物素化的抗人SDF-1/CXCL12抗体和辣根过氧化物酶标记的亲和素,抗人SDF-1/CXCL12抗体与结合在包被抗体上的人SDF-1/CXCL12结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,SDF-1/CXCL12浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中SDF-1/CXCL12的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人SDF-1/CXCL12,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
IF: 11.556 -
Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo
IF: 8.947Journal:Acta Biomaterialia -
Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury
IF: 8.947 -
Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion
IF: 8.469 -
Targeting CXCL12/CXCR4 Signaling with AMD3100 Might Selectively Suppress CXCR4+ T-Cell Chemotaxis Leading to the Alleviation of Chronic Prostatitis
IF: 6.922 -
Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12β/CXCR2/4 signal transduction
IF: 6.53Journal:BIOMEDICINE & PHARMACOTHERAPY -
Anti-Fibrotic Effect of SDF-1β Overexpression in Bleomycin-Injured Rat Lung
IF: 6.525Journal:Pharmaceutics -
Effect of fructooligosaccharide on endothelial function in CKD patients: a randomized controlled trial
IF: 5.992Journal:NEPHROLOGY DIALYSIS TRANSPLANTATION -
Association of serum stromal cell-derived factor-1 levels with EZSCAN score and its derived indicators in patients with type 2 diabetes
IF: 3.335Journal:Endocrine Connections -
Depot-specific inflammation with decreased expression of ATM2 in white adipose tissues induced by high-margarine/lard intake
IF: 2.806Journal:PLoS One