Human sRANKL(Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit
中文名称:人可溶性核因子κB受体活化因子配基(sRANKL)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人sRANKL抗体包被于酶标板上,实验时样品(或标准品)中的人sRANKL会与包被抗体结合。后依次加入生物素化的抗人sRANKL抗体和辣根过氧化物酶标记的亲和素,抗人sRANKL抗体与结合在包被抗体上的人sRANKL结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,sRANKL浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中sRANKL的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人sRANKL,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Single-Cell Transcriptomics Reveals the Complexity of the Tumor Microenvironment of Treatment-Naive Osteosarcoma
IF: 6.244 -
BML-111 inhibits osteoclast differentiation by suppressing the MAPK and NF-κB pathways, alleviating deterioration of the knee joints in a CIA rat model
IF: 4.473Journal:CELL BIOLOGY INTERNATIONAL -
Corydalis Saxicola Bunting Total Alkaloids Attenuate Walker 256-Induced Bone Pain and Osteoclastogenesis by Suppressing RANKL-Induced NF-κB and c-Fos/NFATc1 Pathways in Rats
IF: 4.225 -
Effects of joint irrigation combined with ozone injection on bone metabolism, inflammatory factors, and joint function in knee osteoarthritis.
IF: 3.94Journal:American Journal of Translational ResearchPMID:36777823 -
Evaluation of gingival crevicular fluid and peri-implant crevicular fluid levels of sclerostin, TWEAK, RANKL and OPG
IF: 3.514 -
Assessment of Salivary Levels of RANKL and OPG in Aggressive versus Chronic Periodontitis
IF: 3.404Journal:Journal of Immunology Research -
CR6-interacting factor-1 contributes to osteoclastogenesis by inducing receptor activator of nuclear factor κB ligand after radiation
IF: 3.231Journal:World Journal of Stem Cells -
Effect of Calcium Hydroxide Alone or in Combination with Ibuprofen and Ciprofloxacin on Nuclear Factor Kappa B Ligand and Osteoprotegerin Level in Periapical Lesions: A Randomized Controlled Clinical Study
IF: 2.833Journal:JOURNAL OF ENDODONTICS -
Sera of patients with axial spondyloarthritis (axSpA) enhance osteoclastogenic potential of monocytes isolated from healthy individuals
IF: 1.998 -
RANKL Gene Polymorphism as a Potential Biomarker to Identify Acute Charcot Foot Among Indian Population With Type 2 Diabetes: A Preliminary Report:
IF: 1.277Journal:International Journal of Lower Extremity Wounds