Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称:人肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人TNF-α抗体包被于酶标板上,实验时样品(或标准品)中的人TNF-α会与包被抗体结合。后依次加入生物素化的抗人TNF-α抗体和辣根过氧化物酶标记的亲和素,抗人TNF-α抗体与结合在包被抗体上的人TNF-α结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TNF-α浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TNF-α的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人TNF-α,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Anti-Inflammatory Effects of Four Psilocybin-Containing Magic Mushroom Water Extracts in vitro on 15-Lipoxygenase Activity and on Lipopolysaccharide-Induced Cyclooxygenase-2 and Inflammatory Cytokines in Human U937 Macrophage Cells
IF: 6.922Journal:Journal of Inflammation Research -
MoS2 nanoflower based electrochemical biosensor for TNF alpha detection in cancer patients
IF: 6.901Journal:ELECTROCHIMICA ACTA -
Safety and Effects of Lactobacillus paracasei TISTR 2593 Supplementation on Improving Cholesterol Metabolism and Atherosclerosis-Related Parameters in Subjects with Hypercholesterolemia: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial
IF: 6.706Journal:Nutrients -
Thymoquinone Effect on Monocyte-Derived Macrophages, Cell-Surface Molecule Expression, and Phagocytosis
IF: 6.706Journal:Nutrients -
Relationship between the Dietary Inflammatory Index Score and Cytokine Levels in Chinese Pregnant Women during the Second and Third Trimesters
IF: 6.706Journal:Nutrients -
Alteration in Gut Microbiota Associated with Zinc Deficiency in School-Age Children
IF: 6.706 -
Different oral and gut microbial profiles in those with Alzheimer's disease consuming anti-inflammatory diets.
IF: 6.59Journal:Frontiers in Nutrition -
Simple and facile carbon dots based electrochemical biosensor for TNF-α targeting in cancer patient's sample
IF: 6.558 -
Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo
IF: 6.543 -
Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity
IF: 6.53