Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples)
中文名称:丙二醛(MDA)比色法测试盒(测细胞)
Price:
- 样本类型: 细胞
- 检测仪器及波长: 酶标仪(530-540 nm,最佳测波长532 nm)
- 研究领域: 氧化应激,铁死亡
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
过氧化脂质降解产物中的丙二醛(MDA)在高温及酸性环境下可与硫代巴比妥酸(TBA)反应产生红棕色产物3,5,5’-三甲基恶唑2,4-二酮(三甲川),该物质在532 nm处有最大吸收峰。
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指标简称 | MDA |
灵敏度 | 0.29 nmol/mL |
检测范围 | 0.29-100 nmol/mL |
精密度 | 批间差:3.5% | 批内差:3.3% |
检测时间 | 65 min |
检测仪器及波长 | 酶标仪(530-540 nm,最佳测波长532 nm) |
保存条件 | 该产品可在 2~8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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Q1:Test cell samples, make cell suspension after homogenization, if temporarily unexpected, can it be stored for a few days at most?
It is recommended that cell suspensions that have been homogenized be tested on the same day and not frozen.If there is a temporary accident, you can directly freeze the cells, it is generally recommended that -80℃ not exceed one month.
Q2:Is it normal for reagent 3 to be solid? Can it continue to be used?
It is normal that solid precipitation may occur after storage of reagent 3. It is heated and dissolved at 80℃, and can be used normally after cooling.
Q3:Can the homogenization medium in the kit be replaced by normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)?
The sample homogenate medium of this kit can only be used with reagent 5 as required in the manual, and cannot be replaced.