Mouse Aβ1-42(Amyloid Beta 1-42) ELISA Kit
中文名称:小鼠β淀粉样蛋白1-42(Aβ1-42)酶联免疫吸附测定试剂盒
别称:
Aβ42、Amyloid Beta 42
展开Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 3.13-200 pg/mL
- 灵敏度: 1.88 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠Aβ1-42抗体包被于酶标板上,实验时样品(或标准品)中的小鼠Aβ1-42会与包被抗体结合。后依次加入生物素化的抗小鼠Aβ1-42抗体和辣根过氧化物酶标记的亲和素,抗小鼠Aβ1-42抗体与结合在包被抗体上的小鼠Aβ1-42结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Aβ1-42浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中Aβ1-42的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 3.13-200 pg/mL |
灵敏度 | 1.88 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠Aβ1-42,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Camellia oil improves Aβ25-35-induced memory impairment by regulating the composition of the gut microbiota and lipid metabolism in mice
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Anti-depressive-like and cognitive impairment alleviation effects of Gastrodia elata Blume water extract is related to gut microbiome remodeling in ApoE−/− mice exposed to unpredictable chronic mild stress
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Yeast β-glucan alleviates cognitive deficit by regulating gut microbiota and metabolites in Aβ1–42-induced AD-like mice
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Cnga2 Knockout Mice Display Alzheimer’s-Like Behavior Abnormities and Pathological Changes
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Codonopsis pilosula polysaccharide attenuates Aβ toxicity and cognitive defects in APP/PS1 mice
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Expression of AHI1 Rescues Amyloidogenic Pathology in Alzheimer’s Disease Model Cells
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NPD1 Enhances Autophagy and Reduces Hyperphosphorylated Tau and Amyloid-β 42 by Inhibiting GSK3β Activation in N2a/APP695swe Cells
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Effect of the MAGL/FAAH Dual Inhibitor JZL-195 on Streptozotocin-Induced Alzheimer’s Disease-like Sporadic Dementia in Mice with an Emphasis on Aβ, HSP-70, Neuroinflammation, and Oxidative Stress
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Biophysical, Biochemical, and Behavioral Implications of ApoE3 Conjugated Donepezil Nanomedicine in a Aβ1–42 Induced Alzheimer’s Disease Rat Model
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