Mouse AMH(Anti-Mullerian Hormone) ELISA Kit
中文名称:小鼠抗缪勒管激素(AMH)酶联免疫吸附测定试剂盒
别称:
MIF、MIH、MIS
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 0.78-50 ng/mL
- 灵敏度: 0.47 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠AMH抗体包被于酶标板上,实验时样品(或标准品)中的小鼠AMH会与包被抗体结合。后依次加入生物素化的抗小鼠AMH抗体和辣根过氧化物酶标记的亲和素,抗小鼠AMH抗体与结合在包被抗体上的小鼠AMH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,AMH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中AMH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法 |
检测范围 | 0.78-50 ng/mL |
灵敏度 | 0.47 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠AMH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Bisphenol A promotes autophagy in ovarian granulosa cells by inducing AMPK/mTOR/ULK1 signalling pathway
IF: 7.577 -
The acceleration of reproductive aging in Nrg1flox/flox;Cyp19‐Cre female mice
IF: 6.714Journal:AGING CELL -
Vinorelbine Potently Induces Placental Cell Death, Does Not Harm Fertility and is a Potential Treatment for Ectopic Pregnancy
IF: 6.183Journal:EBioMedicine -
Melatonin prevents cyclophosphamide-induced primordial follicle loss by inhibiting ovarian granulosa cell apoptosis and maintaining AMH expression
IF: 6.055 -
Ubiquitin-like modifier 1 ligating enzyme 1 relieves cisplatin-induced premature ovarian failure by reducing endoplasmic reticulum stress in granulosa cells
IF: 5.211 -
Distinctions in PCOS induced by letrozole versus dehydroepiandrosterone with high-fat diet in mouse model
IF: 5.051Journal:ENDOCRINOLOGY -
The effect of Bu Shen Huo Xue Tang on autoimmune premature ovarian insufficiency via Modulation of the Nrf2/Keap1 signaling pathway in mice
IF: 4.36 -
Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice
IF: 4.285Journal:BIOLOGY OF REPRODUCTION -
Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles
IF: 3.998Journal:Scientific Reports -
Hydroxychloroquine protects against autoimmune premature ovarian insufficiency by modulating the Treg/Th17 cell ratio in BALB/c mice
IF: 3.777Journal:AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY