Mouse CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit
中文名称:小鼠Ⅰ型胶原交联羧基端肽(CTXⅠ)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠CTXⅠ抗体包被于酶标板上,实验时样品(或标准品)中的小鼠CTXⅠ会与包被抗体结合。后依次加入生物素化的抗小鼠CTXⅠ抗体和辣根过氧化物酶标记的亲和素,抗小鼠CTXⅠ抗体与结合在包被抗体上的小鼠CTXⅠ结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,CTXⅠ浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中CTXⅠ的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠CTXⅠ,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Neuronal Induction of Bone-Fat Imbalance through Osteocyte Neuropeptide Y
IF: 16.806 -
Extracellular Vesicles from Child Gut Microbiota Enter into Bone to Preserve Bone Mass and Strength
IF: 16.806 -
Site-1 protease controls osteoclastogenesis by mediating LC3 transcription
IF: 15.828 -
Aged bone matrix-derived extracellular vesicles as a messenger for calcification paradox
IF: 14.919 -
Sigma-1 receptor attenuates osteoclastogenesis by promoting ER-associated degradation of SERCA2
IF: 14.26 -
Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG
IF: 13.567Journal:Bone Research -
Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption
IF: 13.417 -
Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines
IF: 12.354Journal:Bone Research -
HSC70 mediated autophagic degradation of oxidized PRL2 is responsible for osteoclastogenesis and inflammatory bone destruction
IF: 12.067 -
CNC-bZIP protein NFE2L1 regulates osteoclast differentiation in antioxidant-dependent and independent manners
IF: 11.799