Mouse IFNα(Interferon Alpha) ELISA Kit
中文名称:小鼠干扰素α(IFNα)酶联免疫吸附测定试剂盒
别称:
IFL、IFNA、IFN、IFNalpha
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFNα抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IFNα会与包被抗体结合。后依次加入生物素化的抗小鼠IFNα抗体和辣根过氧化物酶标记的亲和素,抗小鼠IFNα抗体与结合在包被抗体上的小鼠IFNα结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IFNα浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFNα的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IFNα,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
FBXO6 regulates the anti-viral immune-responses via mediating alveolar macrophages survival
IF: 20.693 -
Sialic acid conjugate-modified liposomes enable tumor homing of epirubicin via neutrophil/monocyte infiltration for tumor therapy
IF: 8.947 -
Interplay Between Sialic Acids, Siglec-E, and Neu1 Regulates MyD88- and TRIF-Dependent Pathways for TLR4-Activation During Leishmania donovani Infection
IF: 7.561 -
Neutralizing interferon-α blocks inflammation-mediated vascular injury via PI3K and AMPK in systemic lupus erythematosus
IF: 7.397Journal:IMMUNOLOGY -
Antitumor activity of novel POLA1-HDAC11 dual inhibitors
IF: 6.514 -
Differential Effects of Viral Nucleic Acid Sensor Signaling Pathways on Testicular Sertoli and Leydig Cells
IF: 4.736 -
H5N1 avian influenza virus without 80–84 amino acid deletion at the NS1 protein hijacks the innate immune system of dendritic cells for an enhanced mammalian pathogenicity
IF: 4.188Journal:Transboundary and Emerging Diseases -
NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis
IF: 3.594 -
Structural characterization of two endopolysaccharides from Phellinus sp. and their immunologic effects by intragastric administration in a healthy mammalian model
IF: 3.289Journal:Food & Function -
The immune-enhancing effect and in vitro antioxidant ability of different fractions separated from Colla corii asini
IF: 2.72