Mouse IFN-β(Interferon Beta) ELISA Kit
中文名称:小鼠β干扰素(IFN-β)酶联免疫吸附测定试剂盒
别称:
IFNB1、IFB、IFF、IFNB
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFN-β抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IFN-β会与包被抗体结合。后依次加入生物素化的抗小鼠IFN-β抗体和辣根过氧化物酶标记的亲和素,抗小鼠IFN-β抗体与结合在包被抗体上的小鼠IFN-β结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IFN-β浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFN-β的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IFN-β,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
FBXO6 regulates the anti-viral immune-responses via mediating alveolar macrophages survival
IF: 20.693 -
Sensitizing Tumors to Immune Checkpoint Blockage via STING Agonists Delivered by Tumor-Penetrating Neutrophil Cytopharmaceuticals
IF: 18.027 -
ADP-ribosyltransferase PARP11 modulates the interferon antiviral response by mono-ADP-ribosylating the ubiquitin E3 ligase β-TrCP
IF: 14.633Journal:Nature Microbiology -
STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
IF: 11.799 -
Co-delivery of phagocytosis checkpoint and STING agonist by a Trojan horse nanocapsule for orthotopic glioma immunotherapy
IF: 11.6 -
Peptide nanotube loaded with a STING agonist, c-di-GMP, enhance cancer immunotherapy against melanoma
IF: 10.269Journal:Nano Research -
RNF138 inhibits late inflammatory gene transcription through degradation of SMARCC1 of the SWI/SNF complex.
IF: 9.995Journal:Cell Reports -
Sialic acid conjugate-modified liposomes enable tumor homing of epirubicin via neutrophil/monocyte infiltration for tumor therapy
IF: 8.947 -
Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation
IF: 8.025Journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES -
Structural characterization of a soluble polysaccharide SSPS1 from soy whey and its immunoregulatory activity in macrophages
IF: 8.025Journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULESPMID:35835298