Mouse IFN-β(Interferon Beta) ELISA Kit
中文名称:小鼠β干扰素(IFN-β)酶联免疫吸附测定试剂盒
别称:
IFNB1、IFB、IFF、IFNB
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFN-β抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IFN-β会与包被抗体结合。后依次加入生物素化的抗小鼠IFN-β抗体和辣根过氧化物酶标记的亲和素,抗小鼠IFN-β抗体与结合在包被抗体上的小鼠IFN-β结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IFN-β浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFN-β的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IFN-β,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Interplay Between Sialic Acids, Siglec-E, and Neu1 Regulates MyD88- and TRIF-Dependent Pathways for TLR4-Activation During Leishmania donovani Infection
IF: 7.561 -
Ubiquitin E3 ligase MID1 inhibits the innate immune response by ubiquitinating IRF3
IF: 7.397 -
Discovery of fusidic acid derivatives as novel STING inhibitors for treatment of sepsis
IF: 7.088 -
Design and synthesis of hederagenin derivatives modulating STING/NF-κB signaling for the relief of acute liver injury in septic mice
IF: 7.088Journal:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY -
Chitosan-Poly(Acrylic Acid) Nanoparticles Loaded with R848 and MnCl2 Inhibit Melanoma via Regulating Macrophage Polarization and Dendritic Cell Maturation
IF: 6.4Journal:International Journal of Nanomedicine -
Induction of OTUD1 by RNA viruses potently inhibits innate immune responses by promoting degradation of the MAVS/TRAF3/TRAF6 signalosome
IF: 6.158Journal:PLoS Pathogens -
Menthone inhibits type-I interferon signaling by promoting Tyk2 ubiquitination to relieve local inflammation of rheumatoid arthritis
IF: 5.714 -
Activation of the STING-IRF3 pathway involved in psoriasis with diabetes mellitus
IF: 5.31Journal:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE -
The selective STING inhibitor H-151 preserves myocardial function and ameliorates cardiac fibrosis in murine myocardial infarction
IF: 4.932 -
OTUD1 Negatively Regulates Type I IFN Induction by Disrupting Noncanonical Ubiquitination of IRF3
IF: 4.886Journal:JOURNAL OF IMMUNOLOGY