Mouse IL-18(Interleukin 18) ELISA Kit
中文名称:小鼠白介素18(IL-18)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-18抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-18会与包被抗体结合。后依次加入生物素化的抗小鼠IL-18抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-18抗体与结合在包被抗体上的小鼠IL-18结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-18浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-18的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-18,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Eriodictyol and Homoeriodictyol Improve Memory Impairment in Aβ25–35-Induced Mice by Inhibiting the NLRP3 Inflammasome
IF: 4.412Journal:MOLECULES -
Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways
IF: 4.123 -
Effects of 2,4-dichlorophenoxyacetic acid on the expression of NLRP3 inflammasome and autophagy-related proteins as well as the protective effect of Lycium barbarum polysaccharide in neonatal rats
IF: 4.119 -
C1q/tumor necrosis factor-related protein 9 protects cultured chondrocytes from IL-1β-induced inflammatory injury by inhibiting NLRP3 inflammasome activation via the AdipoR1/AMPK axis
IF: 4.119 -
2,4-dichlorophenoxyacetic acid induces ROS activation in NLRP3 inflammatory body-induced autophagy disorder in microglia and the protective effect of Lycium barbarum polysaccharide
IF: 4.119 -
Adiponectin Inhibits NLRP3 Inflammasome Activation in Nonalcoholic Steatohepatitis via AMPK-JNK/ErK1/2-NFκB/ROS Signaling Pathways
IF: 3.9 -
IGF2 inhibits hippocampal over-activated microglia and alleviates depression-like behavior in LPS- treated male mice
IF: 3.715Journal:BRAIN RESEARCH BULLETIN -
Acetaldehyde dehydrogenase 2 activation attenuates sepsis-induced brain injury through NLRP3 inflammasome regulation
IF: 3.715Journal:BRAIN RESEARCH BULLETIN -
Verapamil Ameliorates Hepatic Metaflammation by Inhibiting Thioredoxin-Interacting Protein/NLRP3 Pathways
IF: 3.519Journal:Frontiers in Endocrinology -
NLRP6 Induces Lung Injury and Inflammation Early in Brucella and Influenza Coinfection
IF: 3.508Journal:Journal of Personalized Medicine