Mouse TSH(Thyroid Stimulating Hormone) ELISA Kit
中文名称:小鼠促甲状腺素(TSH)酶联免疫吸附测定试剂盒
别称:
Thyrotropin
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 1.25-80 ng/mL
- 灵敏度: 0.75 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠TSH抗体包被于酶标板上,实验时样品(或标准品)中的小鼠TSH会与包被抗体结合。后依次加入生物素化的抗小鼠TSH抗体和辣根过氧化物酶标记的亲和素,抗小鼠TSH抗体与结合在包被抗体上的小鼠TSH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TSH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TSH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法 |
检测范围 | 1.25-80 ng/mL |
灵敏度 | 0.75 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠TSH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Transdermal Delivery of Anti‐Obesity Compounds to Subcutaneous Adipose Tissue with Polymeric Microneedle Patches
IF: 14.188Journal:Small Methods -
Repositioning of Cefuroxime as novel selective inhibitor of the thyroid hormone activating enzyme type 2 Deiodinase
IF: 10.334Journal:PHARMACOLOGICAL RESEARCH -
TSH Combined with TSHR Aggravates Diabetic Peripheral Neuropathy by Promoting Oxidative Stress and Apoptosis in Schwann Cells
IF: 6.543 -
Chronic exposure to microcystin-LR reduces thyroid hormone levels by activating p38/MAPK and MEK/ERK signal pathway
IF: 6.291 -
Transcriptome Profile of Thyroid Glands in Bile Duct Ligation Mouse Model
IF: 6.208 -
The Thyroid Hormone Inactivator Enzyme, Type 3 Deiodinase, Is Essential for Coordination of Keratinocyte Growth and Differentiation
IF: 5.309 -
Saikosaponin-d Attenuates Hashimoto’s Thyroiditis by Regulating Macrophage Polarization
IF: 4.493Journal:Journal of Immunology Research -
Renal protective effects of grape seed extract treatment against Eltroxin-induced hyperthyroidism, kidney damage, and oxidative stress in male mice
IF: 3.056Journal:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH -
Subclinical hypothyroidism contributes to poor glycemic control in patients with type 2 diabetes mellitus, and ellagic acid attenuates methimazole-induced abnormal glucose metabolism in mice model
IF: 2.72 -
Reduced expression of PD-L1 in autoimmune thyroiditis attenuate trophoblast invasion through ERK/MMP pathway
IF: 2.589