PE/Cyanine5 Anti-Mouse/Human CD11b Antibody[M1/70]
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- 应用性: FCM
- 亚型: Rat IgG2b, κ
- 宿主: Rat
Background | CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. |
Alternate Names | Integrin alpha-M;Itgam;CD11 antigen-like family member B;CR-3 alpha chain;Leukocyte adhesion receptor MO1;CD11b; |
Swissprot | |
Gene ID | |
Clone No | |
Host | Rat |
Reactivity | Human;Mouse |
Application | |
Isotype | Rat IgG2b, κ |
Isotype Control | |
Conjugation | PE/Cyanine 5 |
Conjugation Information | PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter). |
Spectrum | |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA. |
Storage | This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. |
Expiration date | 12个月 |
Shipping |
冰袋 |
Q1:What are the recommendations for flow cytometry of mouse macrophages to detect M1 and M2 type macrophages?
As a recommandation, F4/80 and CD11b are always used for identyfing macrophages of mouse, and CD86 and CD206 can be used for a further seperation for M1 abd M2 respectively. Specific indicators can be determined according to the literature and experimental requirements.