Rat ADP/Acrp30(Adiponectin) ELISA Kit
中文名称:大鼠脂联素(ADP/Acrp30)酶联免疫吸附测定试剂盒
别称:
Acrp30、GBP28、ACDC、APM1、ADPN、AdipoQ、ADIPQTL1
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠ADP/Acrp30抗体包被于酶标板上,实验时样品(或标准品)中的大鼠ADP/Acrp30会与包被抗体结合。后依次加入生物素化的抗大鼠ADP/Acrp30抗体和辣根过氧化物酶标记的亲和素,抗大鼠ADP/Acrp30抗体与结合在包被抗体上的大鼠ADP/Acrp30结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,ADP/Acrp30浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中ADP/Acrp30的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | 比色法 |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠ADP/Acrp30,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Acetate circumvents impaired metabolic switch in skeletal muscle of letrozole-induced PCOS rat model by suppression of PDK4/NLRP3
IF: 4.893Journal:NUTRITION -
Fish oil and corn oil induced differential effect on beiging of visceral and subcutaneous white adipose tissue in high-fat-diet-induced obesity
IF: 4.873Journal:JOURNAL OF NUTRITIONAL BIOCHEMISTRY -
Increased growth hormone secretagogue receptor-1a (GHSR-1a) in hypothalamus during olanzapine treatment in rats
IF: 4.693 -
6-gingerol ameliorates weight gain and insulin resistance in metabolic syndrome rats by regulating adipocytokines
IF: 4.562Journal:SAUDI PHARMACEUTICAL JOURNAL -
Bee bread mitigates downregulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet
IF: 4.31Journal:AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISMPMID:34229480 -
Pine nut oil supplementation alleviates the obesogenic effects in high-fat diet induced obese rats: A comparative study between epididymal and retroperitoneal adipose tissue.
IF: 3.876Journal:NUTRITION RESEARCH -
Synergistic effects of the capsaicinoid nonivamide and rosuvastatin on obesity‐related endothelial dysfunction in rat fed a high‐fat diet
IF: 3.766Journal:PHYTOTHERAPY RESEARCHDOI:10.1002/ptr.6369 -
Curcumin nanoemulsion counteracts hepatic and cardiac complications associated with high-fat/high-fructose diet in rats
IF: 3.654 -
Antiobesity Effect of Flaxseed Polysaccharide via Inducing Satiety due to Leptin Resistance Removal and Promoting Lipid Metabolism through the AMP-Activated Protein Kinase (AMPK) Signaling Pathway
IF: 3.571Journal:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY -
Low dose spironolactone-mediated androgen-adiponectin modulation alleviates endocrine-metabolic disturbances in letrozole-induced PCOS
IF: 3.347
E-OSEL-R0006 | QuicKey Pro Rat ADP/Acrp30(Adiponectin) ELISA Kit | 96T , 48T , 96T*5 |