Rat ADP/Acrp30(Adiponectin) ELISA Kit
中文名称:大鼠脂联素(ADP/Acrp30)酶联免疫吸附测定试剂盒
别称:
Acrp30、GBP28、ACDC、APM1、ADPN、AdipoQ、ADIPQTL1
展开Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠ADP/Acrp30抗体包被于酶标板上,实验时样品(或标准品)中的大鼠ADP/Acrp30会与包被抗体结合。后依次加入生物素化的抗大鼠ADP/Acrp30抗体和辣根过氧化物酶标记的亲和素,抗大鼠ADP/Acrp30抗体与结合在包被抗体上的大鼠ADP/Acrp30结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,ADP/Acrp30浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中ADP/Acrp30的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠ADP/Acrp30,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance
IF: 3.292 -
Effects of combination of obesity, diabetes, and hypoxia on inflammatory regulating genes and cytokines in rat pancreatic tissues and serum
IF: 3.061 -
Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS
IF: 2.861Journal:MOLECULES -
Orlistat attenuates obesity‐induced decline in steroidogenesis and spermatogenesis by up‐regulating steroidogenic genes
IF: 2.86Journal:Andrology -
High fat diet-induced inflammation and oxidative stress are attenuated by N-acetylneuraminic acid in rats
IF: 2.763Journal:JOURNAL OF BIOMEDICAL SCIENCE -
Neonatal zingerone protects against the development of high-fructose diet-induced metabolic syndrome in adult Sprague-Dawley rats
IF: 2.456Journal:Journal of Developmental Origins of Health and Disease -
Investigation of therapeutic potential of three endemic Cirsium species for global health problem obesity
IF: 2.315Journal:SOUTH AFRICAN JOURNAL OF BOTANY -
Combined treatment with interleukin-1 and tumor necrosis factor-alpha antagonists improve type 2 diabetes in rats
IF: 2.21Journal:CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY -
Evaluating the effects of Juglans regia L. extract on hyperglycaemia and insulin sensitivity in experimental type 2 diabetes in rat
IF: 2.11Journal:ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY -
Possible mechanisms mediating the protective effect of cilostazol in L-arginine induced acute pancreatitis in rats: role of cGMP, cAMP, and HO-1
IF: 2.05
E-OSEL-R0006 | QuicKey Pro Rat ADP/Acrp30(Adiponectin) ELISA Kit | 96T , 48T , 96T*5 |