Rat EPO(Erythropoietin) ELISA Kit
中文名称:大鼠促红细胞生成素(EPO)酶联免疫吸附测定试剂盒
别称:
EP、MVCD2、Epoetin、Hematopoietin、Hemopoietin
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 62.5-4000 pg/mL
- 灵敏度: 37.5 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠EPO抗体包被于酶标板上,实验时样品(或标准品)中的大鼠EPO会与包被抗体结合。后依次加入生物素化的抗大鼠EPO抗体和辣根过氧化物酶标记的亲和素,抗大鼠EPO抗体与结合在包被抗体上的大鼠EPO结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,EPO浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中EPO的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 62.5-4000 pg/mL |
灵敏度 | 37.5 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠EPO,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Selective HIF stabilization alleviates hepatocellular steatosis and ballooning in a rodent model of 70% liver resection
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Effect of Zinc Supplementation on Renal Anemia in 5/6-Nephrectomized Rats and a Comparison with Treatment with Recombinant Human Erythropoietin
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The effects of Momordica balsamina methanolic extract on haematological function in streptozotocin-induced diabetic rats: Effects on selected markers
IF: 3.743Journal:BIOMEDICINE & PHARMACOTHERAPY -
Ameliorative Effects of Bovine Lactoferrin on Benzene-Induced Hematotoxicity in Albino Rats
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In anemia zinc is recruited from bone and plasma to produce new red blood cells
IF: 3.212 -
Heamanetic Effects of a Dioxidovanadium(V) Complex in STZ-Induced Diabetic Male Sprague Dawley Rats
IF: 3.168Journal:Diabetes Metabolic Syndrome and Obesity-Targets and TherapyPMID:34707382 -
Telfairia occidentalis Hook.f. - associated haematopoietic effect is mediated by cytokines but independent of testosterone: A preliminary report
IF: 3.115 -
The Haematological Effects of Oleanolic Acid in Streptozotocin-Induced Diabetic Rats: Effects on Selected Markers
IF: 3.04