Rat IFN-γ(Interferon Gamma) ELISA Kit
中文名称:大鼠γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
别称:
IFNG、IFG、IFI、Type II Interferon
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IFN-γ抗体包被于酶标板上,实验时样品(或标准品)中的大鼠IFN-γ会与包被抗体结合。后依次加入生物素化的抗大鼠IFN-γ抗体和辣根过氧化物酶标记的亲和素,抗大鼠IFN-γ抗体与结合在包被抗体上的大鼠IFN-γ结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IFN-γ浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | 比色法 |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠IFN-γ,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Effects of catechin on a rodent model of autism spectrum disorder: implications for the role of nitric oxide in neuroinflammatory pathway
IF: 4.53 -
BMSC-EVs overexpressing PD-L1 ameliorate DSS-induced ulcerative colitis in rats by regulating Th17/Treg cell balance through PTEN/PI3K/AKT/mTOR axis
IF: 4.369Journal:JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY -
LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway
IF: 4.119 -
hUC-MSCs exosomal miR-451 alleviated acute lung injury by modulating macrophage M2 polarization via regulating MIF-PI3K-AKT signaling pathway
IF: 4.109Journal:ENVIRONMENTAL TOXICOLOGY -
MSCs ameliorates DPN induced cellular pathology via [Ca2+]i homeostasis and scavenging the pro‐inflammatory cytokines
IF: 4.08Journal:JOURNAL OF CELLULAR PHYSIOLOGY -
MiRNA-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis
IF: 3.967Journal:APOPTOSIS -
Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome
IF: 3.923 -
NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis
IF: 3.594 -
Inhibiting Succinate Dehydrogenase by Dimethyl Malonate Alleviates Brain Damage in a Rat Model of Cardiac Arrest
IF: 3.59 -
Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity
IF: 3.457Journal:BIOMEDICINE & PHARMACOTHERAPY