Rat INS(Insulin) ELISA Kit
中文名称:大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
别称:
IDDM2、ILPR、IRDN、MODY10
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 6.25-400 pg/mL
- 灵敏度: 3.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上,实验时样品(或标准品)中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素,抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,INS浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中INS的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | 比色法 |
检测范围 | 6.25-400 pg/mL |
灵敏度 | 3.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的大鼠INS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Effects of Casein, Chicken, and Pork Proteins on the Regulation of Body Fat and Blood Inflammatory Factors and Metabolite Patterns Are Largely Dependent on the Protein Level and Less Attributable to the Protein Source
IF: 4.192 -
Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways
IF: 4.162 -
Comparative Biochemical Profiling of Aluminum Chloride and Sodium Azide Induced Neuroinflammation and Cardiometabolic Disturbance
IF: 4.132 -
Therapeutic Potential of Selenium Nanoparticles on Letrozole-Induced Polycystic Ovarian Syndrome in Female Wistar Rats
IF: 4.081Journal:BIOLOGICAL TRACE ELEMENT RESEARCH -
Vanillin improves glucose homeostasis and modulates metabolic activities linked to type 2 diabetes in fructose–streptozotocin induced diabetic rats
IF: 4.076Journal:ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY -
Ezetimibe attenuates experimental diabetes and renal pathologies via targeting the advanced glycation, oxidative stress and AGE-RAGE signalling in rats
IF: 4.076 -
Titin and CK2α are New Intracellular Targets in Acute Insulin Application-Associated Benefits on Electrophysiological Parameters of Left Ventricular Cardiomyocytes From Insulin-Resistant Metabolic Syndrome Rats
IF: 4.069 -
Topical Application of Chinese Formula Yeliangen Promotes Wound Healing in Streptozotocin-Induced Diabetic Rats
IF: 4.061Journal:Journal of Diabetes Research -
Cyclovirobuxine D protects against diabetic cardiomyopathy by activating Nrf2-mediated antioxidant responses
IF: 3.998Journal:Scientific Reports -
Hyperglycemia alters mitochondrial respiration efficiency and mitophagy in human podocytes
IF: 3.905