Rat INS(Insulin) ELISA Kit
中文名称:大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
别称:
IDDM2、ILPR、IRDN、MODY10
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 6.25-400 pg/mL
- 灵敏度: 3.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上,实验时样品(或标准品)中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素,抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,INS浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中INS的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | 比色法 |
检测范围 | 6.25-400 pg/mL |
灵敏度 | 3.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的大鼠INS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Effects of noopept on cognitive functions and pubertal process in rats with diabetes
IF: 3.448 -
Role of simvastatin and insulin in memory protection in a rat model of diabetes mellitus and dementia
IF: 3.44 -
The Roles of Liver Inflammation and the Insulin Signaling Pathway in PM2.5 Instillation-Induced Insulin Resistance in Wistar Rats
IF: 3.434 -
Metabolomic profiling to reveal the therapeutic potency of Posidonia oceanica nanoparticles in diabetic rats
IF: 3.361Journal:RSC Advances -
Bone Marrow-Derived Mesenchymal Stem Cells Restored High-Fat-Fed Induced Hyperinsulinemia in Rats at Early Stage of Type 2 Diabetes Mellitus:
IF: 3.341Journal:CELL TRANSPLANTATION -
Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways
IF: 3.292 -
Supplementation of pyrroloquinoline quinone with atorvastatin augments mitochondrial biogenesis and attenuates low grade inflammation in obese rats
IF: 3.263Journal:EUROPEAN JOURNAL OF PHARMACOLOGY -
Novel sedentary cage induced sedentariness in rats: evidence from relevant biomarkers
IF: 3.263Journal:BMC Endocrine Disorders -
Lingguizhugan Decoction Targets Intestinal Microbiota and Metabolites to Reduce Insulin Resistance in High-Fat Diet Rats
IF: 3.249Journal:Diabetes Metabolic Syndrome and Obesity-Targets and TherapyPMID:35971521 -
Bisacurone attenuates diabetic nephropathy by ameliorating oxidative stress, inflammation and apoptosis in rats
IF: 3.247Journal:HUMAN & EXPERIMENTAL TOXICOLOGY