Rat LEP(Leptin) ELISA Kit
中文名称:大鼠瘦素(LEP)酶联免疫吸附测定试剂盒
别称:
LEPD、OB、OBS、Obesity Homolog
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LEP抗体包被于酶标板上,实验时样品(或标准品)中的大鼠LEP会与包被抗体结合。后依次加入生物素化的抗大鼠LEP抗体和辣根过氧化物酶标记的亲和素,抗大鼠LEP抗体与结合在包被抗体上的大鼠LEP结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,LEP浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中LEP的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠LEP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Maternal Diabetes and Postnatal High-Fat Diet on Pregnant Offspring.
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Hypolipidemic effect of ethanol extract from Chimonanthus nitens Oliv. leaves in hyperlipidemia rats via activation of the leptin/JAK2/STAT3 pathway
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Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model
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Hydroethanolic Extract of A. officinarum Hance Ameliorates Hypertension and Causes Diuresis in Obesogenic Feed-Fed Rat Model
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L-Methionine supplementation attenuates high-fat fructose diet-induced non-alcoholic steatohepatitis by modulating lipid metabolism, fibrosis and inflammation in rats
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Acetate: A therapeutic candidate against renal disorder in a rat model of polycystic ovarian syndrome
IF: 5.011Journal:JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGYPMID:36150640 -
Acetate circumvents impaired metabolic switch in skeletal muscle of letrozole-induced PCOS rat model by suppression of PDK4/NLRP3
IF: 4.893Journal:NUTRITION -
Fish oil and corn oil induced differential effect on beiging of visceral and subcutaneous white adipose tissue in high-fat-diet-induced obesity
IF: 4.873Journal:JOURNAL OF NUTRITIONAL BIOCHEMISTRY -
Increased growth hormone secretagogue receptor-1a (GHSR-1a) in hypothalamus during olanzapine treatment in rats
IF: 4.693 -
6-gingerol ameliorates weight gain and insulin resistance in metabolic syndrome rats by regulating adipocytokines
IF: 4.562Journal:SAUDI PHARMACEUTICAL JOURNAL