Rat LEP(Leptin) ELISA Kit
中文名称:大鼠瘦素(LEP)酶联免疫吸附测定试剂盒
别称:
LEPD、OB、OBS、Obesity Homolog
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LEP抗体包被于酶标板上,实验时样品(或标准品)中的大鼠LEP会与包被抗体结合。后依次加入生物素化的抗大鼠LEP抗体和辣根过氧化物酶标记的亲和素,抗大鼠LEP抗体与结合在包被抗体上的大鼠LEP结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,LEP浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中LEP的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | 比色法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠LEP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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The adverse effects of chronic low-dose exposure to nonylphenol on type 2 diabetes mellitus in high sucrose-high fat diet-treated rats
IF: 2.2Journal:Islets -
Edible Bird’s Nest Prevents High Fat Diet-Induced Insulin Resistance in Rats
IF: 2.164Journal:Journal of Diabetes Research -
N-Acetylneuraminic acid attenuates hypercoagulation on high fat diet-induced hyperlipidemic rats
IF: 2.162Journal:Food & Nutrition Research -
Effect of neonatal orally administered S-allyl cysteine in high-fructose diet fed Wistar rats
IF: 2.07Journal:Journal of Developmental Origins of Health and Disease -
Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones
IF: 1.718 -
The effects of β-escin on inflammation, oxidative stress and Langerhans islet cells in high-fat diet and streptozotocin injection induced experimental type-2 diabetes model
IF: 1.653Journal:BIOLOGIA -
N-Acetylneuraminic Acid Supplementation Prevents High Fat Diet-Induced Insulin Resistance in Rats through Transcriptional and Nontranscriptional Mechanisms
IF: 1.579Journal:Biomed Research International -
Changes of adipocytokine expression after diabetic rats received sitagliptin and the molecular mechanism
IF: 0.841Journal:Asian Pacific Journal of Tropical Medicine -
Combined high dose vitamin C and E increases oxidative stress and visceral fat mass in rats treated by depot-medroxyprogesterone acetate
Journal:Middle East Fertility Society Journal -
Acetate supplementation restores testicular function by modulating Nrf2/PPAR-γ in high fat diet-induced obesity in Wistar rats
Journal:Journal of Diabetes and Metabolic Disorders