Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) ELISA Kit
中文名称:大鼠内皮型一氧化氮合成酶(NOS3/eNOS)酶联免疫吸附测定试剂盒
别称:
ECNOS、NOS Ⅲ、E-NOS
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠NOS3/eNOS抗体包被于酶标板上,实验时样品(或标准品)中的大鼠NOS3/eNOS会与包被抗体结合。后依次加入生物素化的抗大鼠NOS3/eNOS抗体和辣根过氧化物酶标记的亲和素,抗大鼠NOS3/eNOS抗体与结合在包被抗体上的大鼠NOS3/eNOS结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,NOS3/eNOS浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中NOS3/eNOS的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠NOS3/eNOS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Quince extract resists atherosclerosis in rats by down-regulating the EGFR/PI3K/Akt/GSK-3β pathway
IF: 7.419Journal:BIOMEDICINE & PHARMACOTHERAPY -
Renoprotective and therapeutic effects of newly water, ethanol, and butanol ginseng fractions in hypertensive and chronic kidney disease with L-NAME
IF: 6.53 -
D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed rats
IF: 6.53 -
Suppression of PCSK9/NF-kB-dependent pathways by acetate ameliorates cardiac inflammation in a rat model of polycystic ovarian syndrome
IF: 5.037 -
Nitric oxide modulates tapentadol antinociceptive tolerance and physical dependence
IF: 4.432 -
Diet-induced prediabetes: Effects on the activity of the renin-angiotensin-aldosterone system (RAAS) in selected organs
IF: 4.232Journal:Journal of Diabetes Investigation -
Diet-induced prediabetes: effects of exercise treatment on risk factors for cardiovascular complications
IF: 4.169 -
Evaluation of the Cardiovascular Effects of Coriandrum sativum and Citrus limon to Treat Arsenic-Induced Endothelial Damage and Hypertension in Rats
IF: 3.251Journal:Life-Basel -
Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers
IF: 2.538 -
Protective effect of magnesium acetyltaurate and taurine against NMDA-induced retinal damage involves reduced nitrosative stress
IF: 2.219Journal:MOLECULAR VISIONPMID:30090013