Rat NSE(Neuron Specific Enolase) ELISA Kit
中文名称:大鼠神经特异性烯醇化酶(NSE)酶联免疫吸附测定试剂盒
别称:
ENO2、HEL-S-279、NSE、Enolase 2
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 0.31-20 ng/mL
- 灵敏度: 0.19 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠NSE抗体包被于酶标板上,实验时样品(或标准品)中的大鼠NSE会与包被抗体结合。后依次加入生物素化的抗大鼠NSE抗体和辣根过氧化物酶标记的亲和素,抗大鼠NSE抗体与结合在包被抗体上的大鼠NSE结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,NSE浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中NSE的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 0.31-20 ng/mL |
灵敏度 | 0.19 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠NSE,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
A Nanotherapy of Octanoic Acid Ameliorates Cardiac Arrest/Cardiopulmonary Resuscitation-Induced Brain Injury via RVG29- and Neutrophil Membrane-Mediated Injury Relay Targeting
IF: 18.027 -
Inhaled Carbon Dioxide Improves Neurological Outcomes by Downregulating Hippocampal Autophagy and Apoptosis in an Asphyxia‐Induced Cardiac Arrest and Resuscitation Rat Model
IF: 6.106Journal:Journal of the American Heart Association -
Neuroprotective Effect of the Inhibitor Salubrinal after Cardiac Arrest in a Rodent Model
IF: 5.076 -
Cerebral Blood Flow–Guided Manipulation of Arterial Blood Pressure Attenuates Hippocampal Apoptosis After Asphyxia‐Induced Cardiac Arrest in Rats
IF: 4.605Journal:Journal of the American Heart Association -
Triptolide Improves Cognitive Dysfunction in Rats with Vascular Dementia by Activating the SIRT1/PGC-1α Signaling Pathway
IF: 3.996Journal:NEUROCHEMICAL RESEARCH -
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway
IF: 3.503 -
Cathodal tDCS exerts neuroprotective effect in rat brain after acute ischemic stroke.
IF: 2.811Journal:BMC NEUROSCIENCE -
Mitochondrial dynamics and protective effects of a mitochondrial division inhibitor, Mdivi-1, in lipopolysaccharide-induced brain damage
IF: 2.559Journal:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS -
Electroacupuncture reduces astrocyte number and oxidative stress in aged rats with surgery-induced cognitive dysfunction:
IF: 1.351Journal:JOURNAL OF INTERNATIONAL MEDICAL RESEARCH -
Protective role of astragalus injection in spinal cord ischemia-reperfusion injury in rats.
IF: 0.929Journal:Neurosciences