Rat OC/BGP(Osteocalcin) ELISA Kit
中文名称:大鼠骨钙素(OC/BGP)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 0.78-50 ng/mL
- 灵敏度: 0.47 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠OC/BGP抗体包被于酶标板上,实验时样品(或标准品)中的大鼠OC/BGP会与包被抗体结合。后依次加入生物素化的抗大鼠OC/BGP抗体和辣根过氧化物酶标记的亲和素,抗大鼠OC/BGP抗体与结合在包被抗体上的大鼠OC/BGP结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,OC/BGP浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中OC/BGP的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.78-50 ng/mL |
灵敏度 | 0.47 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠OC/BGP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Immunology and bioinformatics analysis of injectable organic/inorganic microfluidic microspheres for promoting bone repair
IF: 15.304 -
Remote-controllable bone-targeted delivery of estradiol for the treatment of ovariectomy-induced osteoporosis in rats
IF: 10.435 -
Urine-derived stem cells-extracellular vesicles ameliorate diabetic osteoporosis through HDAC4/HIF-1α/VEGFA axis by delivering microRNA-26a-5p
IF: 6.691 -
miR-27a-5p—Abundant Small Extracellular Vesicles Derived From Epimedium-Preconditioned Bone Mesenchymal Stem Cells Stimulate Osteogenesis by Targeting Atg4B-Mediated Autophagy
IF: 6.684Journal:Frontiers in Cell and Developmental Biology -
Investigating the Effects of Diet-Induced Pre-Diabetes on the Functioning of Calcium-Regulating Organs in Male Sprague Dawley Rats: Effects on Selected Markers
IF: 6.055 -
Decellularization and oxidation process of bamboo stem enhance biodegradation and osteogenic differentiation
IF: 5.88Journal:Materials Science & Engineering C-Materials for Biological ApplicationsPMID:33321600 -
Glycine Acts Through Estrogen Receptor Alpha to Mediate Estrogen Receptor Signaling, Stimulating Osteogenesis and Attenuating Adipogenesis in Ovariectomized Rats
IF: 5.82Journal:MOLECULAR NUTRITION & FOOD RESEARCH -
Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism
IF: 5.555 -
Aptamer selection and aptasensor construction for bone density biomarkers
IF: 5.339 -
Selenium nanoparticles stimulate osteoblast differentiation via BMP-2/MAPKs/β-catenin pathway in diabetic osteoporosis
IF: 5.307